Difucosylation of chitooligosaccharides by eukaryote and prokaryote α1,6-fucosyltransferases

• Published in: Biochimica et biophysica acta Vol. 1830; no. 10; pp. 4482 - 4490
• Main Authors: Ihara, Hideyuki, Hanashima, Shinya, Tsukamoto, Hiroki, Yamaguchi, Yoshiki, Taniguchi, Naoyuki, Ikeda, Yoshitaka
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2013
• Subjects: Base Sequence; Carbohydrate Sequence; Chitin - metabolism; Chitooligosaccharide; chitooligosaccharides; DNA Primers; eukaryotic cells; Fucose - metabolism; Fucosylation; Fucosyltransferase; Fucosyltransferases - metabolism; Glycosidase; high performance liquid chromatography; hydrolysis; Lysozyme; Magnetic Resonance Spectroscopy; Mass Spectrometry; Molecular Sequence Data; nuclear magnetic resonance spectroscopy; Oligosaccharides - chemistry; Oligosaccharides - metabolism; prokaryotic cells; GNFF; MS; MALDI; GN2; TOCSY; TOF; Fucosyltransferase; GN1; GN4; GN3; COSY; GN6; GN5; GDP; NMR; Fuc; Chitooligosaccharide; Fucosylation; Glycosidase; HSQC; GNF; HPLC; Lysozyme; N,N′,N″,N‴,N‴′-pentaacetyl chitopentaose; difucosylated chitooligosaccharide; hetero-nuclear single quantum coherence; N,N′,N″-triacetyl chitotriose; guanine nucleotide diphosphate; N,N′,N″,N‴,N‴′,N‴″-hexaacetyl chitohexaose; mass spectrometry; correlation spectroscopy; N,N′,N″,N‴-tetraacetyl chitotetraose; fucose; time of flight; FUT8-monofucosylated chitooligosaccharide; GlcNAc or N-acetylglucosamine; nuclear magnetic resonance; high performance liquid chromatography; N,N′-diacetyl chitobiose; NodZ-monofucosylated chitooligosaccharide; matrix-assisted laser desorption/ionization; total correlation spectroscopy

The synthesis of eukaryotic N-glycans and the rhizobia Nod factor both involve α1,6-fucosylation. These fucosylations are catalyzed by eukaryotic α1,6-fucosyltransferase, FUT8, and rhizobial enzyme, NodZ. The two enzymes have similar enzymatic properties and structures but display different acceptor...