Dietary supplementation with increasing doses of an organic micromineral complex on juvenile Nile tilapia: Effects on the antioxidant defense system and tissue deposition

• Published in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Vol. 260; p. 111039
• Main Authors: da Silva Pierri, Bruno, Dafré, Alcir Luiz, Silva, Allan David, Cadorin, Douglas Ismael, Mattos, Jacó Joaquim, Bascunãn, Vera Lúcia Azzolin Frescura, Pettigrew, James E., Fracalossi, Débora Machado
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2021
• Subjects: Animal Feed; Animals; Antioxidants - chemistry; Antioxidants - metabolism; Aquaculture; Brazil; Catalase; Catalase - metabolism; Cichlids - metabolism; Cichlids - physiology; Diet; Dietary Supplements; Glutathione; Glutathione Peroxidase - metabolism; Hepatopancreas - metabolism; Iron - chemistry; Male; Metallothionein; Metallothionein - chemistry; Metallothionein - metabolism; Minerals - metabolism; Oxidative stress; Sulfhydryl Compounds - metabolism; Superoxide Dismutase - metabolism; Zinc - chemistry; Brazil; Oxidative stress; Catalase; Metallothionein; Aquaculture
• ISSN: 1095-6433; 1531-4332
• DOI: 10.1016/j.cbpa.2021.111039

The effect of increasing amounts (0%, 25%, 50%, 75%, and 100%) of dietary supplementation with an organic micromineral complex (Fe, Zn, Cu, Mn, and Se) on antioxidant defenses and mineral deposition in tissues of Nile tilapia juveniles was evaluated, where 100% supplementation represented the average adopted by the feed industry in Brazil. Fish (initial weight 23.93 ± 0.80 g) were fed until apparent satiation twice a day for 56 days. The maximum deposition of Fe and Zn in the hepatopancreas occurred in fish given approximately 50% supplementation, whereas the deposition of Mn and Se increased linearly with the inclusion of the complex. The activity of catalase and superoxide dismutase in the hepatopancreas decreased in fish fed the 50% dose, when compared to those not receiving mineral supplementation or those receiving higher doses. Glutathione peroxidase (GPx) activity in the hepatopancreas increased as the dietary Se concentration increased. However, the concentration of metallothionein in the hepatopancreas showed an inverse relationship to the increase in dietary supplementation of the organic mineral complex. There was no relationship between the doses of organic micromineral supplementation and the activities of GPx, reduced glutathione, non-protein thiols, or protein carbonylation. However, diets supplemented with 50% to 100% promoted greater GPx activity when compared to the 0% supplemented diet. Supplementation with intermediate doses of organic microminerals, approximately 50% of that used in commercial tilapia diets, promoted the homeostasis of metal metabolism, especially for Fe and Zn. [Display omitted] •Tilapia fingerlings showed a limit in the deposition of iron and zinc in the hepatopancreas.•Metallothionein concentration has an inverse relation with the dietary minerals.•GPx activity in the hepatopancreas increased with the dietary Se concentration.