Molecular and functional characterization of circulating extracellular vesicles from diabetic patients with and without retinopathy and healthy subjects

• Published in: Experimental eye research Vol. 176; pp. 69 - 77
• Main Authors: Mazzeo, Aurora, Beltramo, Elena, Lopatina, Tatiana, Gai, Chiara, Trento, Marina, Porta, Massimo
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.11.2018
• Subjects: Adult; Aged; Angiogenesis; Biomarkers - metabolism; Blood-Retinal Barrier - physiology; Capillary Permeability; Cells, Cultured; Diabetes; Diabetes Mellitus, Type 1 - blood; Diabetic retinopathy; Diabetic Retinopathy - blood; Extracellular vesicles; Extracellular Vesicles - physiology; Female; Flow Cytometry; Gene Expression Profiling; Healthy Volunteers; Humans; Male; Microarray Analysis; MicroRNAs - genetics; Middle Aged; miR-150-5p; miR-21-3p; miR-30b-5p; Pericytes; Pericytes - metabolism; Real-Time Polymerase Chain Reaction; CTR; Diabetic retinopathy; HMEC; PS; VEGF; HRP; Extracellular vesicles; HIF-1α; miR-21-3p; VEGFR1/2; DR; ECM; Angiogenesis; EV; miR-30b-5p; Pericytes; KEGG; miRNA; Diabetes; MSC; noDR; miR-150-5p; EC

Diabetic retinopathy is a sight-threatening complication of diabetes, characterized by loss of retinal pericytes and abnormal angiogenesis. We previously demonstrated that extracellular vesicles (EVs) derived from mesenchymal stem cells cultured in diabetic-like conditions are able to enter the peri...