Functional analysis of miR-21-3p, miR-30b-5p and miR-150-5p shuttled by extracellular vesicles from diabetic subjects reveals their association with diabetic retinopathy

• Published in: Experimental eye research Vol. 184; pp. 56 - 63
• Main Authors: Mazzeo, Aurora, Lopatina, Tatiana, Gai, Chiara, Trento, Marina, Porta, Massimo, Beltramo, Elena
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.07.2019
• Subjects: Adult; Aged; Angiogenesis; Biomarkers; Blotting, Western; Cell Movement; Diabetes Mellitus, Type 1 - genetics; Diabetes Mellitus, Type 1 - physiopathology; Diabetic retinopathy; Diabetic Retinopathy - genetics; Diabetic Retinopathy - physiopathology; Extracellular vesicles; Extracellular Vesicles - physiology; Female; Gene Expression Profiling; HIF-1α; Humans; Hypoxia; Hypoxia-Inducible Factor 1, alpha Subunit - genetics; Male; MicroRNAs - genetics; Middle Aged; miR-150-5p; miR-21-3p; miR-30b-5p; Real-Time Polymerase Chain Reaction; RNA, Messenger - genetics; Transfection; Diabetic retinopathy; Angiogenesis; miR-30b-5p; Extracellular vesicles; Hypoxia; HIF-1α; miR-21-3p; miR-150-5p

Microvascular dysfunctions due to altered interactions between endothelial cells (ECs) and pericytes are key-events in the pathogenesis of diabetic retinopathy. Extracellular vesicles (EVs) derived from mesenchymal stem cells cultured in diabetic-like conditions enter pericytes, cause their detachme...