Aldolase-regulated G3BP1/2+ condensates control insulin mRNA storage in beta cells

• Published in: The EMBO journal Vol. 44; no. 13; pp. 3669 - 3696
• Main Authors: Quezada, Esteban, Knoch, Klaus-Peter, Vasiljevic, Jovana, Seiler, Annika, Pal, Akshaye, Gunasekaran, Abishek, Münster, Carla, Friedland, Daniela, Schöniger, Eyke, Sönmez, Anke, Roch, Pascal, Wegbrod, Carolin, Ganß, Katharina, Kipke, Nicole, Alberti, Simon, Nano, Rita, Piemonti, Lorenzo, Aust, Daniela, Weitz, Jürgen, Distler, Marius, Solimena, Michele
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.07.2025
• Subjects: Animals; Biomedical and Life Sciences; Cell Line, Tumor; DNA Helicases; EMBO32; EMBO36; EMBO57; Fructose-Bisphosphate Aldolase - metabolism; Glucose - metabolism; Glucose - pharmacology; Humans; Insulin - genetics; Insulin - metabolism; Insulin-Secreting Cells - metabolism; Life Sciences; Mice; Poly-ADP-Ribose Binding Proteins - genetics; Poly-ADP-Ribose Binding Proteins - metabolism; RNA Helicases - genetics; RNA Helicases - metabolism; RNA Recognition Motif Proteins - genetics; RNA Recognition Motif Proteins - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Translation; Islet; Diabetes; Insulin; Stress Granules

Upregulation of insulin mRNA translation upon hyperglycemia in pancreatic islet β-cells involves several RNA-binding proteins. Here, we found that G3BP1, a stress granule marker downregulated in islets of subjects with type 2 diabetes, binds to insulin mRNA in glucose concentration-dependent manner....