A simple and rapid method for blood plasma separation driven by capillary force with an application in protein detection

Blood plasma separation is a vital sample pre-treatment procedure for microfluidic devices in blood diagnostics, and it requires reliability and speediness. In this work, we propose a novel and simple method for microvolume blood plasma separation driven by capillary force. Flat-shaped filter membra...

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Published inAnalytical methods Vol. 12; no. 2; pp. 256 - 257
Main Authors Gao, Qingxue, Chang, Yongjia, Deng, Qingmei, You, Hui
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 28.05.2020
Subjects
Blood cells
Blood plasma
Capillaries
Devices
Efficiency
Hematocrit
Humans
Interference fit
Lab-On-A-Chip Devices
Microfluidic Analytical Techniques
Microfluidic devices
Microfluidics
Plasma
Pretreatment
Proteins
Reproducibility of Results
Separation
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Abstract Blood plasma separation is a vital sample pre-treatment procedure for microfluidic devices in blood diagnostics, and it requires reliability and speediness. In this work, we propose a novel and simple method for microvolume blood plasma separation driven by capillary force. Flat-shaped filter membranes combined with hydrophilic narrow capillaries are introduced into devices, in order to reduce the residual volumes of blood plasma. An interference fit is used to ensure no leakage of blood or cells. There is desired trapping efficiency of blood cells in the devices. The method provides high efficiency with a plasma extraction yield of 71.7% within 6 min, using 60 μL of undiluted whole human blood with 45% haematocrit. The influence from structural parameters on the separation kinetics and the dependence of the haematocrit levels on the separation efficiency are also investigated. The total protein detection shows considerable protein recovery of 82.3% in the extracted plasma. Thus, the plasma separation unit with a very simple structure is suitable for integrating into microfluidic devices, presenting promising prospects for clinical diagnostics as well as for point-of-care testing applications. Blood plasma separation is a vital sample pre-treatment procedure for microfluidic devices in blood diagnostics, and it requires reliability and speediness.
AbstractList Blood plasma separation is a vital sample pre-treatment procedure for microfluidic devices in blood diagnostics, and it requires reliability and speediness. In this work, we propose a novel and simple method for microvolume blood plasma separation driven by capillary force. Flat-shaped filter membranes combined with hydrophilic narrow capillaries are introduced into devices, in order to reduce the residual volumes of blood plasma. An interference fit is used to ensure no leakage of blood or cells. There is desired trapping efficiency of blood cells in the devices. The method provides high efficiency with a plasma extraction yield of 71.7% within 6 min, using 60 μL of undiluted whole human blood with 45% haematocrit. The influence from structural parameters on the separation kinetics and the dependence of the haematocrit levels on the separation efficiency are also investigated. The total protein detection shows considerable protein recovery of 82.3% in the extracted plasma. Thus, the plasma separation unit with a very simple structure is suitable for integrating into microfluidic devices, presenting promising prospects for clinical diagnostics as well as for point-of-care testing applications.
Blood plasma separation is a vital sample pre-treatment procedure for microfluidic devices in blood diagnostics, and it requires reliability and speediness. In this work, we propose a novel and simple method for microvolume blood plasma separation driven by capillary force. Flat-shaped filter membranes combined with hydrophilic narrow capillaries are introduced into devices, in order to reduce the residual volumes of blood plasma. An interference fit is used to ensure no leakage of blood or cells. There is desired trapping efficiency of blood cells in the devices. The method provides high efficiency with a plasma extraction yield of 71.7% within 6 min, using 60 μL of undiluted whole human blood with 45% haematocrit. The influence from structural parameters on the separation kinetics and the dependence of the haematocrit levels on the separation efficiency are also investigated. The total protein detection shows considerable protein recovery of 82.3% in the extracted plasma. Thus, the plasma separation unit with a very simple structure is suitable for integrating into microfluidic devices, presenting promising prospects for clinical diagnostics as well as for point-of-care testing applications. Blood plasma separation is a vital sample pre-treatment procedure for microfluidic devices in blood diagnostics, and it requires reliability and speediness.
Author Deng, Qingmei
Chang, Yongjia
You, Hui
Gao, Qingxue
AuthorAffiliation University of Science and Technology of China
Chinese Academy of Sciences
Institute of Intelligent Machines
Guangxi University
Department of Laboratory
Cancer Hospital
School of Mechanical Engineering
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Snippet Blood plasma separation is a vital sample pre-treatment procedure for microfluidic devices in blood diagnostics, and it requires reliability and speediness. In...
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StartPage 256
SubjectTerms Blood cells
Blood plasma
Capillaries
Devices
Efficiency
Hematocrit
Humans
Interference fit
Lab-On-A-Chip Devices
Microfluidic Analytical Techniques
Microfluidic devices
Microfluidics
Plasma
Pretreatment
Proteins
Reproducibility of Results
Separation
Title A simple and rapid method for blood plasma separation driven by capillary force with an application in protein detection
URI https://www.ncbi.nlm.nih.gov/pubmed/32930282
https://www.proquest.com/docview/2407310090
https://search.proquest.com/docview/2442848357
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