Molecular-Genetic PET Imaging Using an HSV1-tk Mutant Reporter Gene with Enhanced Specificity to Acycloguanosine Nucleoside Analogs

• Published in: Journal of Nuclear Medicine Vol. 50; no. 3; pp. 409 - 416
• Main Authors: Najjar, Amer M, Nishii, Ryuichi, Maxwell, David S, Volgin, Andrei, Mukhopadhyay, Uday, Bornmann, William G, Tong, William, Alauddin, Mian, Gelovani, Juri G
• Format: Journal Article
• Language: English
• Published: United States Soc Nuclear Med 01.03.2009; Society of Nuclear Medicine
• Subjects: Animals; Arabinofuranosyluracil - analogs & derivatives; Arabinofuranosyluracil - chemistry; Cell Line, Tumor; Enzymes; Feasibility Studies; Fluorine Radioisotopes; Fluorouracil - analogs & derivatives; Fluorouracil - chemistry; Genes, Reporter; Guanine - analogs & derivatives; Guanine - chemistry; Herpesvirus 1, Human - enzymology; Humans; Kinases; Mice; Mice, Nude; Models, Molecular; Mutation; Neoplasm Transplantation; Positron-Emission Tomography; Protein Binding; Radiopharmaceuticals - chemistry; Signal transduction; Stem cells; Structure-Activity Relationship; Thymidine Kinase - chemistry; Thymidine Kinase - genetics; Thymidine Kinase - metabolism; Transplantation, Heterologous; Tumors

Imaging 2 different molecular-genetic events in a single subject by PET is essential in a variety of in vivo applications. Using herpes simplex virus-1 thymidine kinase (HSV1-tk) mutants with narrower substrate specificities in combination with wild-type HSV1-tk (wtHSV1-tk) would enable differential...