Turbulence Activates Platelet Biogenesis to Enable Clinical Scale Ex Vivo Production

• Published in: Cell Vol. 174; no. 3; pp. 636 - 648.e18
• Main Authors: Ito, Yukitaka, Nakamura, Sou, Sugimoto, Naoshi, Shigemori, Tomohiro, Kato, Yoshikazu, Ohno, Mikiko, Sakuma, Shinya, Ito, Keitaro, Kumon, Hiroki, Hirose, Hidenori, Okamoto, Haruki, Nogawa, Masayuki, Iwasaki, Mio, Kihara, Shunsuke, Fujio, Kosuke, Matsumoto, Takuya, Higashi, Natsumi, Hashimoto, Kazuya, Sawaguchi, Akira, Harimoto, Ken-ichi, Nakagawa, Masato, Yamamoto, Takuya, Handa, Makoto, Watanabe, Naohide, Nishi, Eiichiro, Arai, Fumihito, Nishimura, Satoshi, Eto, Koji
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 26.07.2018
• Subjects: bioreactor; Bioreactors; Blood Platelets - metabolism; Cell Culture Techniques - instrumentation; Cell Culture Techniques - methods; Humans; Hydrodynamics; IGFBP2; Induced Pluripotent Stem Cells - metabolism; iPSC; megakaryocyte; Megakaryocytes - metabolism; Megakaryocytes - physiology; MIF; Nardilysin; platelet; regenerative medicine; shear stress; Thrombopoiesis - physiology; turbulence; shear stress; regenerative medicine; iPSC; IGFBP2; megakaryocyte; platelet; MIF; bioreactor; turbulence; Nardilysin

The ex vivo generation of platelets from human-induced pluripotent cells (hiPSCs) is expected to compensate donor-dependent transfusion systems. However, manufacturing the clinically required number of platelets remains unachieved due to the low platelet release from hiPSC-derived megakaryocytes (hi...