Development of a new extraction technique and HPLC method for the analysis of non-psychoactive cannabinoids in fibre-type Cannabis sativa L. (hemp)

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 143; pp. 228 - 236
• Main Authors: Brighenti, Virginia, Pellati, Federica, Steinbach, Marleen, Maran, Davide, Benvenuti, Stefania
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05.09.2017
• Subjects: Cannabinoids; Cannabis; Cannabis sativa L; Chromatography, High Pressure Liquid; Extraction; Hemp; HPLC; Plant Extracts; Spectrometry, Mass, Electrospray Ionization; CBDA; CBD; CBC; CO2; CBG; DM; EtOH; CBNA; CBN; Cannabinoids; CHCl3; Extraction; PFP; HCOOH; HPLC; CID; CBCA; Hemp; H2O; CBGA; MS; ACN; MAE; PEG; Cannabis sativa L; THCA; MeOH; Δ9-THC; UAE

[Display omitted] •A new HPLC method for non-psychoactive cannabinoids analysis in hemp is proposed.•The fused-core stationary phase remarkably improved the HPLC performance.•The extraction procedure was optimized to obtain a high yield.•The method was fully validated in agreement with ICH guideline...