Metabolism of the herbicide metribuzin by an N-glucosyltransferase from tomato cell cultures

• Published in: Plant science (Limerick) Vol. 74; no. 1; pp. 73 - 80
• Main Authors: Davis, D.G., Olson, P.A., Swanson, H.R., Frear, D.S.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 1991; Elsevier Science
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; callus; cell suspension; Chemical control; Fundamental and applied biological sciences. Psychology; glucoside conjugation; Lycopersicon esculentum; metribuzin; N-glucosyltransferase; Parasitic plants. Weeds; Phytopathology. Animal pests. Plant and forest protection; tissue culture; tomato; Weeds; UDPG; 2,4-D; metribuzin; callus; N-glucosyltransferase; DTT; EtSH; tissue culture; FAB; MS; glucoside conjugation; IAA; TLC; tomato; GR; EDTA; NAA; BAP; Lycopersicon esculentum; cell suspension; MGT; HPLC; Cell culture; Hexosyltransferase; Enzyme; Tissue culture; Metabolism; Herbicide; Vegetable crop; Dicotyledones; Angiospermae; Triazine derivatives; Spermatophyta; Solanaceae; Conjugation

Callus and cell suspension cultures of tomato ( Lycopersicon esculentum L. cv. ‘Sheyenne’) form a glucose conjugate of the herbicide metribuzin, an asymmetric triazinone that inhibits photosynthesis. The responsible enzyme, a soluble UDP-glucose: metribuzin N-glucosyltransferase (MGT) has been isola...