Reducing heterophilic antibody interference in immunoassays using single-chain antibodies

• Published in: Analytical biochemistry Vol. 419; no. 2; pp. 333 - 335
• Main Authors: Baird, Cheryl L., Tan, Ruimin, Fischer, Christopher J., Victry, Kristin D., Zangar, Richard C., Rodland, Karin D.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.12.2011
• Subjects: 60 APPLIED LIFE SCIENCES; Animals; ANTIBODIES; Antibodies, Heterophile - immunology; biomarkers; blood serum; diagnostic techniques; ELISA; ENZYME IMMUNOASSAY; enzyme-linked immunosorbent assay; FUNCTIONALS; HAAA; HAMA; Humans; Immunoassay - methods; Immunoassay interference; Immunoglobulin G - blood; Mice; microarray technology; PATIENTS; POLYPEPTIDES; Protein microarray; scFv; scFv, immunoassay, microarray, ELISA; Single-Chain Antibodies - blood; Single-Chain Antibodies - immunology; TARGETS; HAAA; Protein microarray; HAMA; scFv; Immunoassay interference; ELISA

Sandwich enzyme-linked immunosorbent assay (ELISA) microarrays can simultaneously quantify the levels of multiple diagnostic targets in a biological sample. However, as with traditional ELISA diagnostics, endogenous antibodies in patient sera can cause interference. We demonstrate here that reducing...