Na^＋/K^＋-ATPase α-subunit in swimming crab Portunus trituberculatus： molecular cloning, characterization, and expression under low salinity stress

• Published in: Chinese journal of oceanology and limnology Vol. 33; no. 4; pp. 828 - 837
• Main Author: 韩晓琳 刘萍 高保全 王好锋 段亚飞 徐文斐 陈萍
• Format: Journal Article
• Language: English
• Published: Heidelberg Science Press 01.07.2015; Springer Nature B.V
• Subjects: 3' Untranslated regions; 5' Untranslated Regions; Active transport; Adenosine triphosphate; Amino acid sequence; Amino acid sequences; Amino acids; Analysis; Aquatic crustaceans; ATP; ATP酶; Binding sites; Biology; Cell membranes; Cladistics; Cloning; Complementary DNA; Crabs; Crustaceans; Earth and Environmental Science; Earth Sciences; Enzymes; Hepatopancreas; ions; Marine biology; Marine crustaceans; Membranes; molecular cloning; Molecular weight; muscles; Na+/K+-exchanging ATPase; Nucleotide sequence; Nucleotides; Oceanography; open reading frames; PCR; Phosphorylation; Phylogeny; Polymerase chain reaction; Portunus trituberculatus; potassium; rapid amplification of cDNA ends; reverse transcriptase polymerase chain reaction; RT-PCR分析; Salinity; Salinity effects; salt stress; Sequencing; sodium; Swimming; Transcription; Transmembrane domains; 三疣梭子蟹; 亚单位; 分子克隆; 核苷酸序列分析; 盐胁迫; 逆转录聚合酶链反应; salinity; expression; Na; ATPase; α-subunit; K; cloning

Na^＋/K^＋-ATPases are membrane-associated enzymes responsible for the active transport of Na^＋ and K^＋ ions across cell membranes, generating chemical and electrical gradients. These enzymes＇ α-subunit provides catalytic function, binding and hydrolyzing ATP, and itself becoming phosphorylated during...