Separation and characterization of A- and B-type starch granules in wheat endosperm
Mature wheat (Triticum aestivum L.) endosperm contains two types of starch granules: large A-type and small B-type. Two methods. microsieving or centrifugal sedimentation through aqueous solutions of sucrose, maltose, or Percoll were used to separate A- and B-type starch granules. Microsieving could...
Saved in:
Published in | Cereal chemistry Vol. 76; no. 3; pp. 375 - 379 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
01.05.1999
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | Mature wheat (Triticum aestivum L.) endosperm contains two types of starch granules: large A-type and small B-type. Two methods. microsieving or centrifugal sedimentation through aqueous solutions of sucrose, maltose, or Percoll were used to separate A- and B-type starch granules. Microsieving could not completely separate the two types of starch granules, while centrifuging through maltose and sucrose solutions gave a homogenous population for B-type starch granules only. Centrifuging through two Percoll solutions (70 and 100%, v/v) produced purified populations of both the A- and B-type starch granules. Analysis of starch granule size distribution in the purified A- and B-type granule populations and in the whole-starch granule population obtained directly from wheat endosperm confirmed that the purified A and B-type starch granule populations represented their counterparts in mature wheat endosperm. Centrifugations through two Percoll solutions were used to purify A- and B-type starch granule populations from six wheat cultivars. The amylose concentrations and gelatinization properties of these populations were analyzed. All of the A-type starch granules contained higher amylose concentrations and had higher gelatinization enthalpies than did B-type starch granules. Although A- and B-type starch granules started to gelatinize at a similar temperature, B-type starch granules had higher gelatinization peak and completion temperatures than did A-type starch granules. |
---|---|
AbstractList | Mature wheat (Triticum aestivum L.) endosperm contains two types of starch granules: large A-type and small B-type. Two methods. microsieving or centrifugal sedimentation through aqueous solutions of sucrose, maltose, or Percoll were used to separate A- and B-type starch granules. Microsieving could not completely separate the two types of starch granules, while centrifuging through maltose and sucrose solutions gave a homogenous population for B-type starch granules only. Centrifuging through two Percoll solutions (70 and 100%, v/v) produced purified populations of both the A- and B-type starch granules. Analysis of starch granule size distribution in the purified A- and B-type granule populations and in the whole-starch granule population obtained directly from wheat endosperm confirmed that the purified A and B-type starch granule populations represented their counterparts in mature wheat endosperm. Centrifugations through two Percoll solutions were used to purify A- and B-type starch granule populations from six wheat cultivars. The amylose concentrations and gelatinization properties of these populations were analyzed. All of the A-type starch granules contained higher amylose concentrations and had higher gelatinization enthalpies than did B-type starch granules. Although A- and B-type starch granules started to gelatinize at a similar temperature, B-type starch granules had higher gelatinization peak and completion temperatures than did A-type starch granules. |
Author | Peng, M Hucl, P Chibbar, R.N Gao, M Abdel-Aal, E.S.M |
Author_xml | – sequence: 1 fullname: Peng, M – sequence: 2 fullname: Gao, M – sequence: 3 fullname: Abdel-Aal, E.S.M – sequence: 4 fullname: Hucl, P – sequence: 5 fullname: Chibbar, R.N |
BookMark | eNotjtFKwzAYhYNMsJs-gRfmBVL_5G-T5nKW6YSJF3XXI03StTLT0lREn97ivDp858DhW5JF6IMn5JZDykFn92W53bykXGudKpliiiq_IAnXGTKUWCxIAgCaAebiiixjfJ8RucKEVJUfzGimrg_UBEdtO5Od_Nj9nMu-oWv2Nz2w6XvwNE5mtC09jiZ8nnykXaBfrTcT9cH1cfDjxzW5bMwp-pv_XJH94-at3LLd69Nzud4xmwHkbBbTRgLUvshUrWuV5aCEVtzg7NYUOKN00rlc5rW3WChra-ekzxorhASxInfn38b0B3Mcu3jYVwI4gtAoBBbiF7S5UOw |
CitedBy_id | crossref_primary_10_1016_j_carbpol_2010_05_018 crossref_primary_10_1016_j_cj_2013_07_003 crossref_primary_10_1016_S2095_3119_12_60026_4 crossref_primary_10_1016_j_jfca_2023_105167 crossref_primary_10_1139_cjas_2017_0099 crossref_primary_10_1371_journal_pone_0114342 crossref_primary_10_1016_j_jcs_2009_12_002 crossref_primary_10_1186_s13007_017_0259_2 crossref_primary_10_1016_j_foodhyd_2008_09_016 crossref_primary_10_1016_j_scitotenv_2023_162213 crossref_primary_10_1016_j_cj_2016_05_004 crossref_primary_10_1016_S1671_2927_08_60129_7 crossref_primary_10_1016_j_indcrop_2021_114028 crossref_primary_10_1007_s13197_017_2681_x crossref_primary_10_1080_87559129_2021_2013871 crossref_primary_10_1016_j_foodhyd_2020_106362 crossref_primary_10_1016_S0308_8146_02_00130_9 crossref_primary_10_1016_j_plantsci_2003_10_028 crossref_primary_10_1007_s10681_020_02688_6 crossref_primary_10_1016_j_foodhyd_2016_07_033 crossref_primary_10_1016_j_jfoodeng_2018_11_012 crossref_primary_10_2991_efood_k_210626_001 crossref_primary_10_1016_j_carbpol_2007_05_012 crossref_primary_10_1186_s12870_016_0896_z crossref_primary_10_1111_j_1744_7909_2010_00961_x crossref_primary_10_1007_s11032_015_0371_9 crossref_primary_10_1016_S0733_5210_03_00030_4 crossref_primary_10_1177_1934578X1701200606 crossref_primary_10_1016_j_jcs_2012_07_011 crossref_primary_10_17221_586_2013_PSE crossref_primary_10_17221_231_2021_PSE crossref_primary_10_1016_j_carbpol_2014_08_004 crossref_primary_10_1016_j_lwt_2020_110051 crossref_primary_10_1016_j_carbpol_2018_08_022 crossref_primary_10_1111_j_1467_7652_2005_00163_x crossref_primary_10_1139_g00_046 crossref_primary_10_1186_s12870_019_1643_z crossref_primary_10_1017_S0021859609990554 crossref_primary_10_1007_s11738_010_0478_x crossref_primary_10_1016_j_jcs_2016_11_005 crossref_primary_10_1016_j_foodhyd_2019_02_014 crossref_primary_10_1104_pp_124_1_265 crossref_primary_10_3389_fpls_2017_02252 crossref_primary_10_1007_s00217_009_1196_8 crossref_primary_10_1016_j_foodchem_2006_06_056 crossref_primary_10_1002_star_200800227 crossref_primary_10_1002_star_200800229 crossref_primary_10_1002_star_201300237 crossref_primary_10_3724_SP_J_1006_2008_00632 crossref_primary_10_1002_cyto_a_23261 crossref_primary_10_1094_CCHEM_02_16_0025_R crossref_primary_10_1002_cche_10245 crossref_primary_10_1016_j_carbpol_2006_01_028 crossref_primary_10_4141_cjps2013_065 crossref_primary_10_1016_j_jcs_2017_01_014 crossref_primary_10_1002_jib_187 crossref_primary_10_1002_star_200390025 crossref_primary_10_1007_s40415_015_0169_3 crossref_primary_10_1111_1541_4337_12272 crossref_primary_10_1002_cche_10371 crossref_primary_10_31073_mvis201908_04 crossref_primary_10_1016_j_plaphy_2020_05_043 crossref_primary_10_1111_aab_12509 crossref_primary_10_1002_star_201200232 crossref_primary_10_9787_KJBS_2021_53_2_83 crossref_primary_10_1016_j_foodchem_2003_06_013 crossref_primary_10_1016_j_jcs_2012_01_004 crossref_primary_10_1016_j_lwt_2014_08_017 crossref_primary_10_1002_star_200500417 crossref_primary_10_1002_fbe2_12068 crossref_primary_10_1016_j_jcs_2017_05_004 crossref_primary_10_1016_j_carbpol_2012_01_027 crossref_primary_10_1016_j_carbpol_2022_120169 crossref_primary_10_1016_S2095_3119_14_60782_6 crossref_primary_10_1016_j_plantsci_2022_111452 crossref_primary_10_1016_j_plantsci_2020_110443 crossref_primary_10_1007_s40415_019_00531_4 crossref_primary_10_1016_j_jfca_2017_07_025 crossref_primary_10_1002_star_200300233 crossref_primary_10_1007_s42976_020_00101_5 crossref_primary_10_1016_j_jcs_2013_03_009 crossref_primary_10_1016_j_jprot_2014_08_002 crossref_primary_10_1007_s13197_018_3544_9 crossref_primary_10_1016_j_foodhyd_2004_10_036 crossref_primary_10_1177_1082013212455344 crossref_primary_10_1016_j_carbpol_2008_10_012 crossref_primary_10_1016_j_foodres_2008_07_016 crossref_primary_10_1016_j_jfoodeng_2006_01_086 crossref_primary_10_1016_j_carbpol_2008_08_001 crossref_primary_10_1016_j_plaphy_2020_04_033 crossref_primary_10_1016_S1671_2927_12_60784_6 crossref_primary_10_3724_SP_J_1006_2008_00795 crossref_primary_10_1016_j_foodchem_2014_12_023 crossref_primary_10_1021_pr9010525 crossref_primary_10_1016_j_ijbiomac_2013_09_030 crossref_primary_10_1016_j_jcs_2016_10_011 crossref_primary_10_1111_arcm_12034 crossref_primary_10_3390_molecules27103307 crossref_primary_10_2135_cropsci2016_08_0710 crossref_primary_10_1016_j_jcs_2004_04_005 crossref_primary_10_1016_j_jhevol_2018_02_009 crossref_primary_10_1016_j_foodhyd_2021_106907 crossref_primary_10_3724_SP_J_1006_2008_01092 crossref_primary_10_1016_j_lwt_2024_116411 crossref_primary_10_1016_j_csbj_2023_08_019 crossref_primary_10_1016_j_foodchem_2017_12_054 crossref_primary_10_1002_star_201100093 crossref_primary_10_1016_j_foodchem_2019_01_095 crossref_primary_10_1016_S2095_3119_13_60726_1 crossref_primary_10_3390_ijms21093257 crossref_primary_10_1111_j_1439_037X_2009_00388_x crossref_primary_10_1007_s10068_023_01331_z crossref_primary_10_1016_j_jcs_2010_02_002 crossref_primary_10_1080_10942910903514669 crossref_primary_10_1016_j_foodchem_2006_01_054 crossref_primary_10_1002_star_200300218 crossref_primary_10_1111_ijfs_16515 crossref_primary_10_1111_are_15914 crossref_primary_10_1007_s11053_019_09457_w crossref_primary_10_1002_star_200900166 crossref_primary_10_3390_pr9101836 crossref_primary_10_1016_j_anifeedsci_2005_02_025 crossref_primary_10_1021_jf503999r crossref_primary_10_3390_plants12112192 crossref_primary_10_1016_j_chroma_2006_06_104 crossref_primary_10_1016_j_foodhyd_2015_11_025 crossref_primary_10_1002_jsfa_1746 crossref_primary_10_1016_j_jcs_2009_11_015 crossref_primary_10_1016_j_carbpol_2021_118837 crossref_primary_10_3724_SP_J_1006_2008_00465 crossref_primary_10_1017_S0021859611000475 crossref_primary_10_1002_star_202100065 crossref_primary_10_1016_j_carpta_2021_100080 crossref_primary_10_1016_j_ijbiomac_2019_12_129 crossref_primary_10_1002_star_202100183 crossref_primary_10_1186_s12864_018_5174_z crossref_primary_10_1094_CCHEM_04_15_0085_R crossref_primary_10_1021_jf405424x crossref_primary_10_1111_ijfs_13266 crossref_primary_10_1007_s00217_007_0711_z crossref_primary_10_1111_ijfs_12850 crossref_primary_10_1186_s12870_019_1734_x crossref_primary_10_1016_j_carbpol_2022_119652 crossref_primary_10_1016_S2095_3119_15_61305_3 crossref_primary_10_3389_fpls_2014_00546 crossref_primary_10_1002_star_201200172 crossref_primary_10_1002_star_201800175 crossref_primary_10_1016_j_carbpol_2005_07_021 crossref_primary_10_1002_jib_107 crossref_primary_10_1002_star_201600139 crossref_primary_10_1016_j_jcs_2008_07_011 crossref_primary_10_1017_S0043933910000073 crossref_primary_10_1002_star_201600012 crossref_primary_10_1002_cche_10402 crossref_primary_10_1002_star_201300070 crossref_primary_10_1002_star_201500243 crossref_primary_10_1080_10408398_2021_1992607 crossref_primary_10_1016_j_polymer_2007_11_029 crossref_primary_10_1016_j_carbpol_2018_04_024 crossref_primary_10_1002_j_2050_0416_2007_tb00762_x crossref_primary_10_17221_4083_PSE crossref_primary_10_1016_j_plantsci_2014_12_016 crossref_primary_10_1002_star_200900177 crossref_primary_10_3724_SP_J_1006_2012_01723 crossref_primary_10_1021_jf503865e crossref_primary_10_1016_j_ygeno_2021_06_030 crossref_primary_10_1371_journal_pone_0246095 crossref_primary_10_4141_cjps10039 crossref_primary_10_1021_jf4040767 crossref_primary_10_1007_s00709_013_0485_z crossref_primary_10_1007_s13205_022_03364_3 crossref_primary_10_1016_j_ygeno_2020_05_014 crossref_primary_10_3390_foods9030315 crossref_primary_10_1016_j_carbpol_2010_05_053 crossref_primary_10_1002_star_200800206 crossref_primary_10_1007_s13197_012_0697_9 crossref_primary_10_1016_j_lwt_2022_113166 crossref_primary_10_3389_fpls_2019_00482 crossref_primary_10_1016_j_jcs_2007_09_002 crossref_primary_10_1016_j_sajb_2024_06_004 crossref_primary_10_1006_jcrs_2001_0422 crossref_primary_10_1016_j_foodres_2022_112193 crossref_primary_10_1016_S2095_3119_13_60557_2 crossref_primary_10_1016_j_ijbiomac_2020_08_192 crossref_primary_10_3390_agronomy13061482 crossref_primary_10_1139_cjps_2015_0048 crossref_primary_10_1093_jxb_erx349 crossref_primary_10_3389_fpls_2023_1313640 crossref_primary_10_1016_j_carbpol_2021_118742 crossref_primary_10_1016_j_jcs_2017_08_016 crossref_primary_10_1016_S0168_9452_03_00076_1 crossref_primary_10_1016_S1875_2780_08_60029_2 crossref_primary_10_1016_j_carbpol_2005_01_015 crossref_primary_10_1016_j_jcs_2013_12_011 crossref_primary_10_1371_journal_pone_0147622 crossref_primary_10_1021_jf506349w crossref_primary_10_1111_ijfs_14954 |
ContentType | Journal Article |
DBID | FBQ |
DOI | 10.1094/CCHEM.1999.76.3.375 |
DatabaseName | AGRIS |
DatabaseTitleList | |
Database_xml | – sequence: 1 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
DeliveryMethod | no_fulltext_linktorsrc |
Discipline | Economics Engineering Diet & Clinical Nutrition |
EISSN | 1943-3638 |
EndPage | 379 |
ExternalDocumentID | US201302932238 |
GroupedDBID | -~X 0R~ 1OC 29B 33P 3V. 5GY 6J9 7X2 7XC 8FE 8FG 8FH 8G5 8R4 8R5 AAHHS AAIKC AAJUZ AAMNW AANLZ AASGY AAXRX AAZKR ABCUV ABCVL ABJCF ABJNI ABUWG ACAHQ ACCFJ ACCZN ACGFO ACGFS ACIWK ACPOU ACXBN ACXQS ADBBV ADKYN ADNWM ADOZA ADXAS ADZMN ADZOD AEEZP AEIGN AENEX AEQDE AEUQT AEUYR AFFNX AFFPM AFKRA AFRAH AI. AIURR AIWBW AJBDE ALMA_UNASSIGNED_HOLDINGS ALUQN AMYDB ATCPS AZQEC BENPR BFHJK BGLVJ BHPHI BPHCQ CCPQU CS3 DCZOG DRFUL DRSTM DU5 DWQXO EBS EJD FBQ FRP GNUQQ GUQSH HCIFZ H~9 I4R L6V L7B LATKE LEEKS LUTES LYRES M0K M2O M7S MEWTI O9- OK1 P2P P2W PATMY PQQKQ PROAC PTHSS PYCSY Q2X ROL RWL S0X SJN SUPJJ TAE TWZ U5U UHB VH1 WH7 WOHZO WXSBR XOL ZZTAW ~KM |
ID | FETCH-LOGICAL-c4005-6389a600be847b9b745072971a3317f835076d6dd565bec387ccbdd6e4fc22602 |
ISSN | 0009-0352 |
IngestDate | Wed Dec 27 19:20:08 EST 2023 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 3 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c4005-6389a600be847b9b745072971a3317f835076d6dd565bec387ccbdd6e4fc22602 |
PageCount | 5 |
ParticipantIDs | fao_agris_US201302932238 |
PublicationCentury | 1900 |
PublicationDate | May/June 1999 |
PublicationDateYYYYMMDD | 1999-05-01 |
PublicationDate_xml | – month: 05 year: 1999 text: May/June 1999 |
PublicationDecade | 1990 |
PublicationTitle | Cereal chemistry |
PublicationYear | 1999 |
SSID | ssj0003173 |
Score | 2.0946686 |
Snippet | Mature wheat (Triticum aestivum L.) endosperm contains two types of starch granules: large A-type and small B-type. Two methods. microsieving or centrifugal... |
SourceID | fao |
SourceType | Publisher |
StartPage | 375 |
SubjectTerms | amylose characterization endosperm food processing food processing quality gelation gels particle size separation starch granules wheat |
Title | Separation and characterization of A- and B-type starch granules in wheat endosperm |
Volume | 76 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1dT9swFLUKPGx7QKPbBINNfph4qZy1tRM3j4GBEBJIU6nEG4o_gpBKKkGrSfz6Hdv56gBp20uU2kpT5Z5cn-veey4h3-KxVFooBDmTSYIAZaRZmg8LJsfKjhIjbeGbTVxcJmczcX4dX_d6552spdVSRfrpxbqS_7EqxmBXVyX7D5ZtvhQDOId9cYSFcfwrG09tUO6uMop1I7781BDBjPmpI-b3WkEF3R7HLRao1dynYg1-OW88sKXxmuH3XbJ6bB-c7LCue8K1bjQ4iFZcP1-sfc6UsXOW-V4Cg5NoGrVbrivd6ZlsqvK7tM3tazwoxnhQnY1scJqp4IwnQaWl9qqhq0uFHt5xkTx0SqlWWx5ayTxz5Ig6nUCw04VwBZVpJJOIR821awrZs-nY__8KKgoCskE25MT5utOjn82azOtcg-rX1_pTqfj-wj3AM4q8yzOu3pPtKkCgWbD2DunZsk_2ftzZJT2klYrrnF7WTRT65E1dW_7YJ-868pIfyLTFBwUI6J_4oIuCZsxPBXzQgA9a44PeldTjgzb4-EhmpydXx2esaqLBtHAqs46R5mC1yoKHqFRJETuxeDnKOR5KAQI-lIlJjAGzx_vMJ1JrZUxiRaFBzYfjT2SzXJR2l1A11FYhHtBgMMIgFLXgfkrH4Hwq57HYI7t4bDf5LZanm3WjfH59ap-8bYF2QLYKvJz2C5jeUn31RvwNIvlLHg |
link.rule.ids | 786 |
linkProvider | FAO Food and Agriculture Organization of the United Nations |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Separation+and+characterization+of+A-+and+B-type+starch+granules+in+wheat+endosperm&rft.jtitle=Cereal+chemistry&rft.au=Peng%2C+M&rft.au=Gao%2C+M&rft.au=Abdel-Aal%2C+E.S.M&rft.au=Hucl%2C+P&rft.date=1999-05-01&rft.issn=0009-0352&rft.eissn=1943-3638&rft.volume=76&rft.issue=3&rft.spage=375&rft.epage=379&rft_id=info:doi/10.1094%2FCCHEM.1999.76.3.375&rft.externalDocID=US201302932238 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0009-0352&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0009-0352&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0009-0352&client=summon |