Proteomic analysis of Fusobacterium nucleatum growth in biofilm versus planktonic state
Fusobacterium nucleatum is isolated from both supra‐ and sub‐gingival dental biofilms in humans and has been implicated in the aetiology of periodontitis. Also, this bacterium plays an important role in serious infections in other parts of the body. The aim of this investigation was to study the pro...
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Published in | Molecular oral microbiology Vol. 35; no. 4; pp. 168 - 180 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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01.08.2020
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Abstract | Fusobacterium nucleatum is isolated from both supra‐ and sub‐gingival dental biofilms in humans and has been implicated in the aetiology of periodontitis. Also, this bacterium plays an important role in serious infections in other parts of the body. The aim of this investigation was to study the protein differential expression of F. nucleatum when growing on biofilm, compared to planktonic state, using proteomic analysis by the 2D‐DIGE™ system. In all, 68 proteins were differentially expressed during biofilm growth (1.5‐fold, p < .05), being 20 downexpressed and 31 overexpressed. The repressed proteins belonged to metabolism, biosynthesis and were outer membrane proteins (OMPs); and overexpressed were proteins involved in metabolism, transcription, translation, transport and proteins with unknown function. Also, of the seven enzymes that regulate the synthesis of butyrate, six of them were differentially expressed (overexpressed and downexpressed) when the bacteria were forming biofilms. The enzymatic activities of two of the enzymes in the butyrate pathway were analysed when the bacteria were growing in biofilms or in planktonic growth. All these results confirmed that this metabolic pathway is important in the formation of the biofilm of F. nucleatum and in its pathogenicity, both in the oral cavity and in other locations of the body.
Effect of Fusobacterium nucleatum on the differential expressions of the butyrate pathway (comparison between the biofilm versus planktonic state) |
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AbstractList | Fusobacterium nucleatum
is isolated from both supra‐ and sub‐gingival dental biofilms in humans and has been implicated in the aetiology of periodontitis. Also, this bacterium plays an important role in serious infections in other parts of the body. The aim of this investigation was to study the protein differential expression of
F. nucleatum
when growing on biofilm, compared to planktonic state, using proteomic analysis by the 2D‐DIGE™ system. In all, 68 proteins were differentially expressed during biofilm growth (1.5‐fold,
p
< .05), being 20 downexpressed and 31 overexpressed. The repressed proteins belonged to metabolism, biosynthesis and were outer membrane proteins (OMPs); and overexpressed were proteins involved in metabolism, transcription, translation, transport and proteins with unknown function. Also, of the seven enzymes that regulate the synthesis of butyrate, six of them were differentially expressed (overexpressed and downexpressed) when the bacteria were forming biofilms. The enzymatic activities of two of the enzymes in the butyrate pathway were analysed when the bacteria were growing in biofilms or in planktonic growth. All these results confirmed that this metabolic pathway is important in the formation of the biofilm of
F. nucleatum
and in its pathogenicity, both in the oral cavity and in other locations of the body. Fusobacterium nucleatum is isolated from both supra‐ and sub‐gingival dental biofilms in humans and has been implicated in the aetiology of periodontitis. Also, this bacterium plays an important role in serious infections in other parts of the body. The aim of this investigation was to study the protein differential expression of F. nucleatum when growing on biofilm, compared to planktonic state, using proteomic analysis by the 2D‐DIGE™ system. In all, 68 proteins were differentially expressed during biofilm growth (1.5‐fold, p < .05), being 20 downexpressed and 31 overexpressed. The repressed proteins belonged to metabolism, biosynthesis and were outer membrane proteins (OMPs); and overexpressed were proteins involved in metabolism, transcription, translation, transport and proteins with unknown function. Also, of the seven enzymes that regulate the synthesis of butyrate, six of them were differentially expressed (overexpressed and downexpressed) when the bacteria were forming biofilms. The enzymatic activities of two of the enzymes in the butyrate pathway were analysed when the bacteria were growing in biofilms or in planktonic growth. All these results confirmed that this metabolic pathway is important in the formation of the biofilm of F. nucleatum and in its pathogenicity, both in the oral cavity and in other locations of the body. Effect of Fusobacterium nucleatum on the differential expressions of the butyrate pathway (comparison between the biofilm versus planktonic state) Fusobacterium nucleatum is isolated from both supra‐ and sub‐gingival dental biofilms in humans and has been implicated in the aetiology of periodontitis. Also, this bacterium plays an important role in serious infections in other parts of the body. The aim of this investigation was to study the protein differential expression of F. nucleatum when growing on biofilm, compared to planktonic state, using proteomic analysis by the 2D‐DIGE™ system. In all, 68 proteins were differentially expressed during biofilm growth (1.5‐fold, p < .05), being 20 downexpressed and 31 overexpressed. The repressed proteins belonged to metabolism, biosynthesis and were outer membrane proteins (OMPs); and overexpressed were proteins involved in metabolism, transcription, translation, transport and proteins with unknown function. Also, of the seven enzymes that regulate the synthesis of butyrate, six of them were differentially expressed (overexpressed and downexpressed) when the bacteria were forming biofilms. The enzymatic activities of two of the enzymes in the butyrate pathway were analysed when the bacteria were growing in biofilms or in planktonic growth. All these results confirmed that this metabolic pathway is important in the formation of the biofilm of F. nucleatum and in its pathogenicity, both in the oral cavity and in other locations of the body. |
Author | Llama‐Palacios, Arancha Sánchez, María C. Herrera, David Potupa, Oksana Sanz, Mariano Figuero, Elena |
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Snippet | Fusobacterium nucleatum is isolated from both supra‐ and sub‐gingival dental biofilms in humans and has been implicated in the aetiology of periodontitis.... Fusobacterium nucleatum is isolated from both supra‐ and sub‐gingival dental biofilms in humans and has been implicated in the aetiology of periodontitis.... |
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SubjectTerms | 2D‐DIGE Bacteria biofilm Biofilms Biosynthesis butyrate pathway Dentistry Enzymatic activity Enzymes Fusobacterium nucleatum Membrane proteins Metabolic pathways Metabolism Oral cavity Outer membrane proteins Pathogenicity Pathogens Periodontitis Protein transport Proteins proteomic Proteomics Transcription Two dimensional analysis |
Title | Proteomic analysis of Fusobacterium nucleatum growth in biofilm versus planktonic state |
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