Transforming growth factor β (TGF-β) expression in isolated and cultured rat hepatocytes

• Published in: Journal of cellular physiology Vol. 167; no. 3; pp. 394 - 405
• Main Authors: Gao, Chunfang, Gressner, G., Zoremba, M., Gressner, A. M.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.06.1996
• Subjects: Animals; Base Sequence; Blotting, Western; Cells, Cultured; Culture Media; Electrophoresis, Agar Gel; Gene Expression Regulation - genetics; Immunoblotting; Immunohistochemistry; Liver - cytology; Liver - metabolism; Male; Microscopy, Fluorescence; Molecular Sequence Data; Polymerase Chain Reaction; Radioimmunoassay; Rats; Rats, Sprague-Dawley; RNA, Messenger - genetics; RNA, Messenger - metabolism; Transforming Growth Factor beta - biosynthesis; Transforming Growth Factor beta - genetics

It is still a subject of debate whether hepatocytes have the ability to express TGF‐β. Therefore, we investigated in freshly isolated and in monolayer cultures of rat hepatocytes the expression of TGF‐β isoforms at the RNA and protein level applying RT‐PCR, immunocytochemistry, immunoblotting, and f...