Inhibition of prolyl 4-hydroxylase by oxalyl amino acid derivatives in vitro, in isolated microsomes and in embryonic chicken tissues

• Published in: Biochemical journal Vol. 300; no. 2; pp. 525 - 530
• Main Authors: BAADER, E, TSCHANK, G, BARINGHAUS, K.-H, BURGHARD, H, GÜNZLER, V
• Format: Journal Article
• Language: English
• Published: Colchester Portland Press 01.06.1994
• Subjects: Amino Acid Sequence; Amino Acids - chemistry; Amino Acids - pharmacology; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Chick Embryo; Culture Techniques; Densitometry; Electrophoresis, Polyacrylamide Gel; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Hydroxyproline - biosynthesis; Microsomes - enzymology; Molecular Sequence Data; Oxalates - chemistry; Oxalates - pharmacology; Oxidoreductases; Procollagen-Proline Dioxygenase - antagonists & inhibitors; Procollagen-Proline Dioxygenase - isolation & purification; Spectrometry, Fluorescence; Vertebrata; Enzyme; Aminoacid; Enzyme inhibitor; Chicken; Oxidoreductases; Aves; Microsome; Mechanism of action; In vitro; Procollagen-proline dioxygenase; Biological activity

The potency of oxalyl amino acid derivatives as inhibitors of prolyl 4-hydroxylase was studied in vitro, in isolated microsomes and in chicken embryonic-tissue culture. These compounds represent structural analogues of 2-oxoglutarate in which the -CH2- moiety at C-3 is replaced by -NH-, with or with...