Low-Copy-Number Human Transgene Is Recognized as an X Inactivation Center in Mouse ES Cells, but Fails to Induce cis-Inactivation in Chimeric Mice

• Published in: Genomics (San Diego, Calif.) Vol. 71; no. 2; pp. 156 - 162
• Main Authors: Migeon, Barbara R., Winter, Holly, Kazi, Ethan, Chowdhury, Ashis K., Hughes, Aisha, Haisley-Royster, Camille, Morrison, Harris, Jeppesen, Peter
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 15.01.2001; Elsevier
• Subjects: Animals; Biological and medical sciences; Cells, Cultured; Chimera - genetics; Clone Cells; Dosage Compensation, Genetic; Embryo, Mammalian - cytology; Female; Fetal Death - genetics; Fundamental and applied biological sciences. Psychology; Gene Dosage; Genes. Genome; Humans; Male; Mice; Mice, Transgenic; Molecular and cellular biology; Molecular genetics; RNA - metabolism; RNA, Long Noncoding; RNA, Untranslated - genetics; Stem Cells - metabolism; Transcription Factors - genetics; Transfection; Transgenes - genetics; X chromosome inactivation center; XIC gene; Human; Stem cell; Rodentia; Transgenic animal; Chromosome 6; Gene expression; Inactivation; Vertebrata; Mammalia; Gene; Mouse; Embryonic cell; X-Chromosome
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.2000.6421

X chromosome inactivation is initiated from a segment of the mammalian X chromosome called the X inactivation center. Transgenes from this region of the murine X chromosome are providing the means to identify the DNA needed for cis inactivation in mice. We recently showed that chimeric mice carrying...