Evaluation of Mitochondrial Function in Isolated Rat Hepatocytes and Mitochondria during Oxidative Stress

• Published in: Alternatives to laboratory animals Vol. 35; no. 3; pp. 353 - 361
• Main Authors: Cervinková, Z, Lotková, H, Krivaková, P, Rousar, T, Kucera, O, Tichý, L, Cervinka, M, Drahota, Z
• Format: Journal Article
• Language: English
• Published: England 01.06.2007
• Subjects: Animal Testing Alternatives; Animals; Cells, Cultured; Dose-Response Relationship, Drug; hepatocytes; Hepatocytes - drug effects; Hepatocytes - metabolism; Hepatocytes - pathology; in vitro studies; Male; Manometry; Membrane Potential, Mitochondrial - drug effects; Membrane Potential, Mitochondrial - physiology; mitochondria; Mitochondria, Liver - drug effects; Mitochondria, Liver - metabolism; Mitochondrial Swelling - drug effects; Mitochondrial Swelling - physiology; Oxidants - toxicity; Oxidative Stress; Oxygen - analysis; Oxygen - metabolism; Oxygen Consumption; Rats; Rats, Wistar; tert-Butylhydroperoxide - toxicity

The majority of toxic agents act either fully or partially via oxidative stress, the liver, specifically the mitochondria in hepatocytes, being the main target. Maintenance of mitochondrial function is essential for the survival and normal performance of hepatocytes, which have a high energy require...