Development of translationally active cell lysates from different filamentous fungi for application in cell-free protein synthesis

• Published in: Enzyme and microbial technology Vol. 185; p. 110588
• Main Authors: Friedrich, Stephanie, Schramm, Marina, Kiebist, Jan, Schmidtke, Kai-Uwe, Scheibner, Katrin
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2025
• Subjects: Agrocybe; Aspergillus niger; biomass; catalysts; cell-free protein synthesis; Cell-free protein synthesis (CFPS); Cell-Free System - metabolism; Culture Media; Fungal lysates; Fungal Proteins - biosynthesis; Fungal Proteins - genetics; fungi; Fungi - genetics; Fungi - metabolism; Genes, Reporter; Green Fluorescent Proteins - biosynthesis; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; in vitro translation; luciferase; Luciferases, Firefly - biosynthesis; Luciferases, Firefly - genetics; Neurospora crassa; Neurospora crassa - genetics; Neurospora crassa - metabolism; organic nitrogen; Protein Biosynthesis; space and time; technology; translation (genetics); Unspecific peroxygenase; Neurospora crassa; Cell-free protein synthesis (CFPS); Fungal lysates; in vitro translation; Unspecific peroxygenase

There is an enormous potential for cell-free protein synthesis (CFPS) systems based on filamentous fungi in view of their simple, fast and mostly inexpensive cultivation with high biomass space-time yields and in view of their catalytic capacity. In 12 of the 22 different filamentous fungi examined,...