Development of translationally active cell lysates from different filamentous fungi for application in cell-free protein synthesis - Friedrich, Stephanie; Schramm, Marina; Kiebist, Jan; Schmidtke, Kai-Uwe; Scheibner, Katrin (Enzyme and microbial technology, 2025)
Keywords: Agrocybe; Aspergillus niger; biomass; catalysts; cell-free protein synthesis; Cell-free protein synthesis (CFPS); Cell-Free System - metabolism; Culture Media; Fungal lysates; Fungal Proteins - biosynthesis; Fungal Proteins - genetics; fungi; Fungi - genetics; Fungi - metabolism; Genes, Reporter; Green Fluorescent Proteins - biosynthesis; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; in vitro translation; luciferase; Luciferases, Firefly - biosynthesis; Luciferases, Firefly - genetics; Neurospora crassa; Neurospora crassa - genetics; Neurospora crassa - metabolism; organic nitrogen; Protein Biosynthesis; space and time; technology; translation (genetics); Unspecific peroxygenase; Neurospora crassa; Cell-free protein synthesis (CFPS); Fungal lysates; in vitro translation; Unspecific peroxygenase