Modulation of Ca2+ oscillation following ischemia and nicotinic acetylcholine receptors in primary cortical neurons by high-throughput analysis

• Published in: Scientific reports Vol. 14; no. 1; pp. 27667 - 18
• Main Authors: Sasaki, Tsutomu, Hisada, Sunao, Kanki, Hideaki, Nunomura, Kazuto, Lin, Bangzhong, Nishiyama, Kumiko, Kawano, Tomohito, Matsumura, Shigenobu, Mochizuki, Hideki
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 12.11.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/154; 631/154/1438; 631/154/570; 631/378/1689; 631/378/340; 631/378/87; Acetylcholine receptors (nicotinic); Agonists; Allosteric properties; Ca2+ oscillations; Calcium antagonists; Calcium influx; Calcium signalling; Dizocilpine; Drug development; Drug screening; Glutamate receptors; Glutamic acid receptors (ionotropic); High-throughput screening; Humanities and Social Sciences; Inhibitory postsynaptic potentials; Ischemia; multidisciplinary; N-Methyl-D-aspartic acid receptors; nAChR; Neuromodulation; Neuroprotection; Neuroprotective agents; PAM; Science; Science (multidisciplinary); α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid; α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors; High-throughput screening; Ischemia; oscillations; PAM; nAChR; Ca

Calcium oscillations in primary neuronal cultures and iPSCs have been employed to investigate arrhythmogenicity and epileptogenicity in drug development. Previous studies have demonstrated that Ca 2+ influx via NMDA and nicotinic acetylcholine receptors (nAChRs) modulates Ca 2+ oscillations. Neverth...