Synergistic effects of hyaluronate - epidermal growth factor conjugate patch on chronic wound healing

The proteolytic microenvironment in the wound area reduces the stability and the half-life of growth factors in vivo , making difficult the topical delivery of growth factors. Here, epidermal growth factor (EGF) was conjugated to hyaluronate (HA) to improve the long-term stability against enzymatic...

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Published inBiomaterials science Vol. 6; no. 5; pp. 12 - 13
Main Authors Kim, Yun Seop, Sung, Dong Kyung, Kong, Won Ho, Kim, Hyemin, Hahn, Sei Kwang
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 01.05.2018
Subjects
Biological effects
Conjugates
Epidermal growth factor
Growth factors
Receptors
Skin
Stability
Sustained release
Wound healing
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Abstract The proteolytic microenvironment in the wound area reduces the stability and the half-life of growth factors in vivo , making difficult the topical delivery of growth factors. Here, epidermal growth factor (EGF) was conjugated to hyaluronate (HA) to improve the long-term stability against enzymatic degradation and the therapeutic effect by enhancing the biological interaction with HA receptors on skin cells. After the synthesis of HA-EGF conjugates, they were incorporated into a patch-type formulation for the facile topical application and sustained release of EGF. According to ELISA, the HA-EGF conjugates showed a long-term stability compared with native EGF. Furthermore, HA-EGF conjugates appeared to interact with skin cells through two types of HA and EGF receptors, resulting in a synergistically improved healing effect. Taken together, we could confirm the feasibility of HA-EGF conjugates for the transdermal treatment of chronic wounds. A HA-EGF conjugate patch was developed for the treatment of diabetic chronic wounds with advantages such as facilitating topical delivery and interaction with dual receptors, and alleviating the degradation of EGF.
AbstractList The proteolytic microenvironment in the wound area reduces the stability and the half-life of growth factors in vivo, making difficult the topical delivery of growth factors. Here, epidermal growth factor (EGF) was conjugated to hyaluronate (HA) to improve the long-term stability against enzymatic degradation and the therapeutic effect by enhancing the biological interaction with HA receptors on skin cells. After the synthesis of HA-EGF conjugates, they were incorporated into a patch-type formulation for the facile topical application and sustained release of EGF. According to ELISA, the HA-EGF conjugates showed a long-term stability compared with native EGF. Furthermore, HA-EGF conjugates appeared to interact with skin cells through two types of HA and EGF receptors, resulting in a synergistically improved healing effect. Taken together, we could confirm the feasibility of HA-EGF conjugates for the transdermal treatment of chronic wounds.
The proteolytic microenvironment in the wound area reduces the stability and the half-life of growth factors in vivo , making difficult the topical delivery of growth factors. Here, epidermal growth factor (EGF) was conjugated to hyaluronate (HA) to improve the long-term stability against enzymatic degradation and the therapeutic effect by enhancing the biological interaction with HA receptors on skin cells. After the synthesis of HA–EGF conjugates, they were incorporated into a patch-type formulation for the facile topical application and sustained release of EGF. According to ELISA, the HA–EGF conjugates showed a long-term stability compared with native EGF. Furthermore, HA–EGF conjugates appeared to interact with skin cells through two types of HA and EGF receptors, resulting in a synergistically improved healing effect. Taken together, we could confirm the feasibility of HA–EGF conjugates for the transdermal treatment of chronic wounds.
The proteolytic microenvironment in the wound area reduces the stability and the half-life of growth factors in vivo , making difficult the topical delivery of growth factors. Here, epidermal growth factor (EGF) was conjugated to hyaluronate (HA) to improve the long-term stability against enzymatic degradation and the therapeutic effect by enhancing the biological interaction with HA receptors on skin cells. After the synthesis of HA-EGF conjugates, they were incorporated into a patch-type formulation for the facile topical application and sustained release of EGF. According to ELISA, the HA-EGF conjugates showed a long-term stability compared with native EGF. Furthermore, HA-EGF conjugates appeared to interact with skin cells through two types of HA and EGF receptors, resulting in a synergistically improved healing effect. Taken together, we could confirm the feasibility of HA-EGF conjugates for the transdermal treatment of chronic wounds. A HA-EGF conjugate patch was developed for the treatment of diabetic chronic wounds with advantages such as facilitating topical delivery and interaction with dual receptors, and alleviating the degradation of EGF.
Author Sung, Dong Kyung
Kong, Won Ho
Kim, Hyemin
Kim, Yun Seop
Hahn, Sei Kwang
AuthorAffiliation Department of Pediatrics
School of Medicine
Department of Materials Science and Engineering
Pohang University of Science and Technology (POSTECH)
Sungkyunkwan University
Samsung Medical Center
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Snippet The proteolytic microenvironment in the wound area reduces the stability and the half-life of growth factors in vivo , making difficult the topical delivery of...
The proteolytic microenvironment in the wound area reduces the stability and the half-life of growth factors in vivo, making difficult the topical delivery of...
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SubjectTerms Biological effects
Conjugates
Epidermal growth factor
Growth factors
Receptors
Skin
Stability
Sustained release
Wound healing
Title Synergistic effects of hyaluronate - epidermal growth factor conjugate patch on chronic wound healing
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