A cell fractionation approach for the quantitative analysis of subcellular drug disposition

• Published in: Pharmaceutical research Vol. 21; no. 1; pp. 26 - 32
• Main Authors: Duvvuri, Muralikrishna, Feng, Wenhong, Mathis, Amanda, Krise, Jeffrey P
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 01.01.2004
• Subjects: Cell Fractionation - methods; Cell Nucleus - chemistry; Cell Nucleus - metabolism; Cytosol - chemistry; Cytosol - metabolism; Doxorubicin - analysis; Doxorubicin - pharmacokinetics; Drug Evaluation, Preclinical - methods; Fluorescent Dyes - analysis; Fluorescent Dyes - pharmacokinetics; Humans; Subcellular Fractions - chemistry; Subcellular Fractions - metabolism; U937 Cells

The purpose of this work was to develop and validate a method that can be used to quantify drugs associated with intracellular compartments. The human leukemic cell line U-937 was used to evaluate the distribution of model compounds with known and different subcellular distribution profiles. Lysotra...