Measurement of the Water Permeability of the Membranes of Boar, Ram, and Rabbit Spermatozoa Using Concentration-Dependent Self-Quenching of an Entrapped Fluorophore

• Published in: Cryobiology Vol. 41; no. 2; pp. 167 - 173
• Main Authors: Curry, M.R., Kleinhans, F.W., Watson, P.F.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.09.2000; Elsevier
• Subjects: Animals; Biological and medical sciences; carboxyfluorescein; Cell Membrane Permeability; Cell Size; Cryopreservation; Fluoresceins - metabolism; Fluorescent Dyes - metabolism; Fluorometry - methods; fluorophores; Fundamental and applied biological sciences. Psychology; Male; Mammalian male genital system; Models, Theoretical; Morphology. Physiology; Osmolar Concentration; Rabbits - physiology; Semen Preservation; Sheep - physiology; Species Specificity; Spermatozoa - metabolism; Spermatozoa - ultrastructure; Swine - physiology; Vertebrates: reproduction; Water - metabolism; Spermatozoa; Semen; Rabbit; Male; Concentration; Germinal cell; Lagomorpha; Water permeability; Pig; Vertebrata; Mammalia; Fluorophore; Animal; Plasma membrane; Self quenching; Sheep; Artiodactyla; Ungulata

Published values for sperm membrane water permeability (Lp) obtained using a time-to-lysis methodology have produced anomalous results when used to model optimal cooling rates for cryopreservation of spermatozoa. As the lysis method is dependent on potentially questionable assumptions, we describe a...