Detection of EGFR Gene Mutation in Lung Cancer by Mutant-Enriched Polymerase Chain Reaction Assay

• Published in: Clinical cancer research Vol. 12; no. 1; pp. 43 - 48
• Main Authors: ASANO, Hiroaki, TOYOOKA, Shinichi, HIRAKI, Akio, SUGI, Kazuro, KIURA, Katsuyuki, DATE, Hiroshi, SHIMIZU, Nobuyoshi, TOKUMO, Masaki, ICHIMURA, Kouichi, AOE, Keisuke, ITO, Sachio, TSUKUDA, Kazunori, OUCHIDA, Mamoru, AOE, Motoi, KATAYAMA, Hideki
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 01.01.2006
• Subjects: Antineoplastic agents; Biological and medical sciences; Biopsy; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - pathology; CT-guided needle lung biopsy; DNA Mutational Analysis; DNA Primers; EGFR; Female; Genes, erbB-1 - genetics; Humans; Lung Neoplasms - genetics; Lung Neoplasms - pathology; Medical sciences; mutant-enriched PCR; Mutation; NSCLC; Pharmacology. Drug treatments; Pleural Effusion; pleural fluid; Pneumology; Polymerase Chain Reaction - methods; Sensitivity and Specificity; Tumors of the respiratory system and mediastinum; Lung disease; Respiratory disease; Lung cancer; Malignant tumor; Bronchopulmonary; Epidermal growth factor receptor; Polymerase chain reaction; Gene; Bronchus disease; Genetics; Diagnosis; Mutation; Molecular biology; Detection

Purpose: Mutations in the epidermal growth factor receptor ( EGFR ) gene have been reported to be present in non–small cell lung cancer (NSCLC) and related to the responsiveness of tumors to EGFR tyrosine kinase inhibitors, suggesting its usefulness as a biomarker. Because clinical samples contain t...