Characteristic of slow growth in cell culture of adenovirus type 54 causing nationwide outbreak epidemic keratoconjunctivitis in Japan

• Published in: Japanese journal of ophthalmology Vol. 64; no. 3; pp. 312 - 320
• Main Authors: Tsukahara-Kawamura, Tomoko, Hanaoka, Nozomu, Konagaya, Masami, Uchio, Eiichi, Fujimoto, Tsuguto
• Format: Journal Article
• Language: English
• Published: Tokyo Springer Japan 01.05.2020; Springer Nature B.V
• Subjects: A549 Cells - virology; Adenovirus E1 Proteins - genetics; Adenovirus Infections, Human - epidemiology; Adenovirus Infections, Human - virology; Adenoviruses; Adenoviruses, Human - classification; Adenoviruses, Human - physiology; Cell culture; Cell Culture Techniques; Cell Line; Cell lines; Cytopathogenic Effect, Viral; Disease Outbreaks; DNA Copy Number Variations; DNA, Viral - genetics; E1 gene; Epidemics; Eye Infections, Viral - epidemiology; Eye Infections, Viral - virology; Growth rate; Humans; Japan - epidemiology; Keratoconjunctivitis; Keratoconjunctivitis - epidemiology; Keratoconjunctivitis - virology; Laboratory Investigation; Medicine; Medicine & Public Health; Open reading frames; Ophthalmology; Outbreaks; Phenotypes; Phylogenetics; Phylogeny; Propagation; Replication initiation; RNA, Messenger - genetics; Transcription initiation; Viral Load; Virus Cultivation; Japan; Species D; Epidemic keratoconjunctivitis; Type 54; Adenovirus; Slow propagation

Purpose To characterize the virological features of adenovirus type 54 (Ad54) causing nationwide outbreak of severe epidemic keratoconjunctivitis (EKC) in Japan, we comparatively analysed the viral propagation phenotype of Ad54 and other Ads: type 37 (Ad37), 64 (Ad64), and 5 (Ad5), in A549 cells qua...