Effects of ascorbate on myogenesis in micromass culture

• Published in: In vitro cellular & developmental biology Vol. 26; no. 3 Pt 1; p. 259
• Main Authors: Archer, C W, Cottrill, C P, Lee, D
• Format: Journal Article
• Language: English
• Published: United States 01.03.1990
• Subjects: Animals; Ascorbic Acid - pharmacology; Cartilage - cytology; Cell Differentiation - drug effects; Cells, Cultured; Chick Embryo; Culture Media; Glycosaminoglycans - biosynthesis; Muscles - cytology; Muscles - drug effects; Muscles - metabolism

Micromass cultures from stage 23 and 24 chick wing mesenchyme were grown in serum-containing medium with or without additional ascorbic acid. It was found that ascorbic acid administered as a single pulse or present continuously throughout culture, in concentrations as low as 25 micrograms/ml, was s...