Phosphorylation of PBX2, a novel downstream target of mTORC1, is determined by GSK3 and PP1

• Published in: Journal of biochemistry (Tokyo) Vol. 173; no. 2; pp. 129 - 138
• Main Authors: Wada, Reona, Fujinuma, Shun, Nakatsumi, Hirokazu, Matsumoto, Masaki, Nakayama, Keiichi I
• Format: Journal Article
• Language: English
• Published: England 03.02.2023
• Subjects: Glycogen Synthase Kinase 3 - metabolism; Mechanistic Target of Rapamycin Complex 1 - metabolism; Phosphorylation; Protein Phosphatase 1 - metabolism; Signal Transduction; TOR Serine-Threonine Kinases - metabolism; mechanistic target of rapamycin complex 1 (mTORC1); phosphorylation; pre–B cell leukemia transcription factor 2 (PBX2); protein phosphatase 1 (PP1); glycogen synthase kinase 3 (GSK3) Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; DMSO, dimethyl sulfoxide; ERK, extracellular signal–regulated kinase; FOXK1, forkhead box K1; GSK3, glycogen synthase kinase 3; HA, hemagglutinin; LARP1, La-related protein 1; MEK, ERK kinase; mTORC1, mechanistic target of rapamycin complex 1; PBS, phosphate-buffered saline; PBX2, pre–B cell leukemia transcription factor 2; PI3K, phosphoinositide 3-kinase; PDK1, phosphoinositide-dependent protein kinase 1; PP1, protein phosphatase 1; PP2A, protein phosphatase 2A; RAG, RAS-related GTP-binding protein; RHEB, Ras homolog enriched in Brain; shRNA, short hairpin RNA; siRNA, small interfering RNA; TBC1D7, TBC1 (TRE2-BUB2-CDC16) domain family member 7; TSC2, tuberous sclerosis complex 2; WT, wild-type

Mechanistic target of rapamycin complex 1 (mTORC1) is a serine-threonine kinase that is activated by extracellular signals, such as nutrients and growth factors. It plays a key role in the control of various biological processes, such as protein synthesis and energy metabolism by mediating or regula...