IgE specific immune responses to an African grass (Kikuyu, Pennisetum clandestinum)
Summary Kikuyu grass (Pennisetum clandeminum) is widely used as a grazing pasture in Africa and, although it is extensively cultivated as a lawn for sports fields and domestic gardens, its allergenicity has never been studied in vitro. Using an extract of Kikuyu grass pollen, polyacrylamide gel elec...
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| Published in | Clinical and experimental allergy Vol. 23; no. 7; pp. 581 - 586 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford, UK
Blackwell Publishing Ltd
01.07.1993
Blackwell |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0954-7894 1365-2222 |
| DOI | 10.1111/j.1365-2222.1993.tb00897.x |
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| Abstract | Summary
Kikuyu grass (Pennisetum clandeminum) is widely used as a grazing pasture in Africa and, although it is extensively cultivated as a lawn for sports fields and domestic gardens, its allergenicity has never been studied in vitro. Using an extract of Kikuyu grass pollen, polyacrylamide gel electrophoresis. Western blotting and a monoclonal anti‐human IgE antibody, the specific IgE binding, in the serum of 160 allergic and non‐allergic patients in the Cape Town area, to a Kikuyu grass extract was studied. IgE in the sera of 43/104 known grass‐allergic individuals hound to Kikuyu grass on the Western blots. In addition, 4/28 ‘non‐allergic’ control subjects were found to have Kikuyu grass‐specific IgE. Five different profiles of specific IgE reactivity to Kikuyu grass antigens were oberved. In 29/43 patients, IgE bound to two dominant 48 and 70 kD allergens in the Kikuyu extract. Although a degree of cross‐reactivity with Bermuda grass (Cynodon dactylis) was found in immunoabsorption studies, the 48 and 70 kD allergens appear to be unique to Kikuyu grass. Exposure of heparinized blood from Kikuyu grass‐positive patients to the Kikuyu extract stimulated the release of histamine from their basophils in vitro. Kikuyu grass pollen is thus identified as an important aero‐allergen in South Africa. |
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| AbstractList | Kikuyu grass (Pennisetum clandestinum) is widely used as a grazing pasture in Africa and, although it is extensively cultivated as a lawn for sports fields and domestic gardens, its allergenicity has never been studied in vitro. Using an extract of Kikuyu grass pollen, polyacrylamide gel electrophoresis, Western blotting and a monoclonal anti-human IgE antibody, the specific IgE binding, in the serum of 160 allergic and non-allergic patients in the Cape Town area, to a Kikuyu grass extract was studied. IgE in the sera of 43/104 known grass-allergic individuals bound to Kikuyu grass on the Western blots. In addition, 4/28 'non-allergic' control subjects were found to have Kikuyu grass-specific IgE. Five different profiles of specific IgE reactivity to Kikuyu grass antigens were observed. In 29/43 patients, IgE bound to two dominant 48 and 70 kD allergens in the Kikuyu extract. Although a degree of cross-reactivity with Bermuda grass (Cynodon dactylis) was found in immunoabsorption studies, the 48 and 70 kD allergens appear to be unique to Kikuyu grass. Exposure of heparinized blood from Kikuyu grass-positive patients to the Kikuyu extract stimulated the release of histamine from their basophils in vitro. Kikuyu grass pollen is thus identified as an important aero-allergen in South Africa. Kikuyu grass (Pennisetum clandestinum) is widely used as a grazing pasture in Africa and, although it is extensively cultivated as a lawn for sports fields and domestic gardens, its allergenicity has never been studied in vitro. Using an extract of Kikuyu grass pollen, polyacrylamide gel electrophoresis, Western blotting and a monoclonal anti-human IgE antibody, the specific IgE binding, in the serum of 160 allergic and non-allergic patients in the Cape Town area, to a Kikuyu grass extract was studied. IgE in the sera of 43/104 known grass-allergic individuals bound to Kikuyu grass on the Western blots. In addition, 4/28 'non-allergic' control subjects were found to have Kikuyu grass-specific IgE. Five different profiles of specific IgE reactivity to Kikuyu grass antigens were observed. In 29/43 patients, IgE bound to two dominant 48 and 70 kD allergens in the Kikuyu extract. Although a degree of cross-reactivity with Bermuda grass (Cynodon dactylis) was found in immunoabsorption studies, the 48 and 70 kD allergens appear to be unique to Kikuyu grass. Exposure of heparinized blood from Kikuyu grass-positive patients to the Kikuyu extract stimulated the release of histamine from their basophils in vitro. Kikuyu grass pollen is thus identified as an important aero-allergen in South Africa.Kikuyu grass (Pennisetum clandestinum) is widely used as a grazing pasture in Africa and, although it is extensively cultivated as a lawn for sports fields and domestic gardens, its allergenicity has never been studied in vitro. Using an extract of Kikuyu grass pollen, polyacrylamide gel electrophoresis, Western blotting and a monoclonal anti-human IgE antibody, the specific IgE binding, in the serum of 160 allergic and non-allergic patients in the Cape Town area, to a Kikuyu grass extract was studied. IgE in the sera of 43/104 known grass-allergic individuals bound to Kikuyu grass on the Western blots. In addition, 4/28 'non-allergic' control subjects were found to have Kikuyu grass-specific IgE. Five different profiles of specific IgE reactivity to Kikuyu grass antigens were observed. In 29/43 patients, IgE bound to two dominant 48 and 70 kD allergens in the Kikuyu extract. Although a degree of cross-reactivity with Bermuda grass (Cynodon dactylis) was found in immunoabsorption studies, the 48 and 70 kD allergens appear to be unique to Kikuyu grass. Exposure of heparinized blood from Kikuyu grass-positive patients to the Kikuyu extract stimulated the release of histamine from their basophils in vitro. Kikuyu grass pollen is thus identified as an important aero-allergen in South Africa. Kikuyu grass ( Pennisetum clandeminum ) is widely used as a grazing pasture in Africa and, although it is extensively cultivated as a lawn for sports fields and domestic gardens, its allergenicity has never been studied in vitro . Using an extract of Kikuyu grass pollen, polyacrylamide gel electrophoresis. Western blotting and a monoclonal anti‐human IgE antibody, the specific IgE binding, in the serum of 160 allergic and non‐allergic patients in the Cape Town area, to a Kikuyu grass extract was studied. IgE in the sera of 43/104 known grass‐allergic individuals hound to Kikuyu grass on the Western blots. In addition, 4/28 ‘non‐allergic’ control subjects were found to have Kikuyu grass‐specific IgE. Five different profiles of specific IgE reactivity to Kikuyu grass antigens were oberved. In 29/43 patients, IgE bound to two dominant 48 and 70 kD allergens in the Kikuyu extract. Although a degree of cross‐reactivity with Bermuda grass ( Cynodon dactylis ) was found in immunoabsorption studies, the 48 and 70 kD allergens appear to be unique to Kikuyu grass. Exposure of heparinized blood from Kikuyu grass‐positive patients to the Kikuyu extract stimulated the release of histamine from their basophils in vitro . Kikuyu grass pollen is thus identified as an important aero‐allergen in South Africa. Summary Kikuyu grass (Pennisetum clandeminum) is widely used as a grazing pasture in Africa and, although it is extensively cultivated as a lawn for sports fields and domestic gardens, its allergenicity has never been studied in vitro. Using an extract of Kikuyu grass pollen, polyacrylamide gel electrophoresis. Western blotting and a monoclonal anti‐human IgE antibody, the specific IgE binding, in the serum of 160 allergic and non‐allergic patients in the Cape Town area, to a Kikuyu grass extract was studied. IgE in the sera of 43/104 known grass‐allergic individuals hound to Kikuyu grass on the Western blots. In addition, 4/28 ‘non‐allergic’ control subjects were found to have Kikuyu grass‐specific IgE. Five different profiles of specific IgE reactivity to Kikuyu grass antigens were oberved. In 29/43 patients, IgE bound to two dominant 48 and 70 kD allergens in the Kikuyu extract. Although a degree of cross‐reactivity with Bermuda grass (Cynodon dactylis) was found in immunoabsorption studies, the 48 and 70 kD allergens appear to be unique to Kikuyu grass. Exposure of heparinized blood from Kikuyu grass‐positive patients to the Kikuyu extract stimulated the release of histamine from their basophils in vitro. Kikuyu grass pollen is thus identified as an important aero‐allergen in South Africa. Kikuyu grass (Pennisetum clandestinum) is widely used as a grazing pasture in Africa and, although it is extensively cultivated as a lawn for sports fields and domestic gardens, its allergenicity has never been studied in vitro. Using an extract of Kikuyu grass pollen, polyacrylamide gel electrophoresis, Western blotting and a monoclonal anti-human IgE antibody, the specific IgE binding, in the serum of 160 allergic and non-allergic patients in the Cape Town area, to a Kikuyu grass extract was studied. Although a degree of cross-reactivity with Bermuda grass (Cynodon dactylis) was found in immunoabsorption studies, the 48 and 70 kD allergens appear to be unique to Kikuyu grass. Exposure of heparinized blood from Kikuyu grass-positive patients to the Kikuyu extract stimulated the release of histamine from their basophils in vitro. Kikuyu grass pollen is thus identified as an important aero-allergen in South Africa. |
| Author | POTTER, P. C. AINSLIE, G. MATHER, S. LOCKEY, P. CADMAN, A. |
| Author_xml | – sequence: 1 givenname: P. C. surname: POTTER fullname: POTTER, P. C. organization: Department of Clinical Science and Immunology, University of Cape Town, Johannesburg, South Africa – sequence: 2 givenname: S. surname: MATHER fullname: MATHER, S. organization: Respiratory Clinic, Department of Medicine, University of Cape Town, Johannesburg, South Africa – sequence: 3 givenname: P. surname: LOCKEY fullname: LOCKEY, P. organization: Allergy Society of South Africa, Johannesburg, South Africa – sequence: 4 givenname: G. surname: AINSLIE fullname: AINSLIE, G. organization: Respiratory Clinic, Department of Medicine, University of Cape Town, Johannesburg, South Africa – sequence: 5 givenname: A. surname: CADMAN fullname: CADMAN, A. organization: Palynology Laboratory, BPI for Palaeontolugical Research, University of Witwatersrand, Johannesburg, South Africa |
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| Cites_doi | 10.1073/pnas.76.9.4350 10.1016/0003-2697(83)90245-2 10.1016/S0254-6299(16)31169-3 10.1016/0091-6749(91)90184-P 10.1111/j.1365-2222.1992.tb00152.x 10.1016/0091-6749(89)90489-2 |
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| References | Van Ree R, Clemens JG, Aalbers M, Stapel SO, Aalberse R. Characterization with monoclonal and polyclonal antibodies of a new major allergen from grass pollen in the group I molecular weight range. J Allergy din Immunol 1989; 83:144-51. Mercer MJ, van Niekcrk CH. Clinical characteristics of childhood asthma. South Afr Med J 1991; 79:77-9. Klysner S, Welinder KG, Lowenstein H. Malthiesen Group V allergens in grass pollens. IV. Similarities in ammo acid and compositions and NH2 terminal sequences of the Group V allergens from Lolium perenne, Poa pratensis and Dactylis glomerulata. Clin Exp Allergy 1992; 22:491-7. Potter PC, Berman D, Toerien A, Malherbe D, Weinberg EG. Clincal significance of aeroallergen identification in the Western Cape. South Afr Med J 1991; 79:80-4. Matthiesen F, Schumacher M, Lowenstein H. Characterization of the major allergen Cynodon dactylon (Bermuda Grass) pollen Cyn d 1. J Allergy Clin Immunol 1991; 88: 763-74. Gibbs-Russel GE, Watson L, Koekemoer M et al. In: Leistner OA, ed. Grasses of southern Africa. South Africa : National Botanic Gardens. Botanical Research Institute, 1991:1-2. Schübeler K. Comparison of different radioimmunoassays (RIA) and radio enzymatic assays (REA). Allergy 1988; 43 (Suppl 7):71. Towbin H, Stachelin T, Gordon J. Elcctrophorctic transfer of proteins from polyacrylamide gels to nitrocellulose sheets, procedure and some applications. Proc Natl Acad Sci USA 1979; 76:4350-4. De Blas A, Cherwinski HM. Detection of antigens on nitrocellulose paper immunoblots with monoclonal antibodies. Anal Biochem 1983; 133:214-9. Watson L, Gibbs-Russel GE, Dallwitz MJ. Grass genera of Southern Africa: interactive identification and information retrieval from an automated databank. S Afr J Bot 1989; 55:452-63. 1988; 43 1974 1989; 83 1989; 55 1983; 133 1991 1991; 79 1992; 22 1979; 76 1991; 88 1979 1955 Scott JD. (e_1_2_1_15_2) 1955 Schübeler K. (e_1_2_1_5_2) 1988; 43 Mercer MJ (e_1_2_1_8_2) 1991; 79 Gibbs‐Russel GE (e_1_2_1_9_2) 1991 e_1_2_1_6_2 e_1_2_1_4_2 e_1_2_1_2_2 e_1_2_1_11_2 e_1_2_1_3_2 e_1_2_1_12_2 e_1_2_1_10_2 e_1_2_1_13_2 e_1_2_1_14_2 Potter PC (e_1_2_1_7_2) 1991; 79 8221253 - Clin Exp Allergy. 1993 Jul;23(7):537-41 |
| References_xml | – reference: Matthiesen F, Schumacher M, Lowenstein H. Characterization of the major allergen Cynodon dactylon (Bermuda Grass) pollen Cyn d 1. J Allergy Clin Immunol 1991; 88: 763-74. – reference: Van Ree R, Clemens JG, Aalbers M, Stapel SO, Aalberse R. Characterization with monoclonal and polyclonal antibodies of a new major allergen from grass pollen in the group I molecular weight range. J Allergy din Immunol 1989; 83:144-51. – reference: De Blas A, Cherwinski HM. Detection of antigens on nitrocellulose paper immunoblots with monoclonal antibodies. Anal Biochem 1983; 133:214-9. – reference: Schübeler K. Comparison of different radioimmunoassays (RIA) and radio enzymatic assays (REA). Allergy 1988; 43 (Suppl 7):71. – reference: Watson L, Gibbs-Russel GE, Dallwitz MJ. Grass genera of Southern Africa: interactive identification and information retrieval from an automated databank. S Afr J Bot 1989; 55:452-63. – reference: Klysner S, Welinder KG, Lowenstein H. Malthiesen Group V allergens in grass pollens. IV. Similarities in ammo acid and compositions and NH2 terminal sequences of the Group V allergens from Lolium perenne, Poa pratensis and Dactylis glomerulata. Clin Exp Allergy 1992; 22:491-7. – reference: Towbin H, Stachelin T, Gordon J. Elcctrophorctic transfer of proteins from polyacrylamide gels to nitrocellulose sheets, procedure and some applications. Proc Natl Acad Sci USA 1979; 76:4350-4. – reference: Potter PC, Berman D, Toerien A, Malherbe D, Weinberg EG. Clincal significance of aeroallergen identification in the Western Cape. South Afr Med J 1991; 79:80-4. – reference: Mercer MJ, van Niekcrk CH. Clinical characteristics of childhood asthma. South Afr Med J 1991; 79:77-9. – reference: Gibbs-Russel GE, Watson L, Koekemoer M et al. In: Leistner OA, ed. Grasses of southern Africa. South Africa : National Botanic Gardens. Botanical Research Institute, 1991:1-2. – volume: 76 start-page: 4350 year: 1979 end-page: 4 article-title: Elcctrophorctic transfer of proteins from polyacrylamide gels to nitrocellulose sheets, procedure and some applications publication-title: Proc Natl Acad Sci USA – volume: 79 start-page: 77 year: 1991 end-page: 9 article-title: Clinical characteristics of childhood asthma publication-title: South Afr Med J – start-page: 653 year: 1955 end-page: 66 – start-page: 724 year: 1955 end-page: 45 – volume: 83 start-page: 144 year: 1989 end-page: 51 article-title: Characterization with monoclonal and polyclonal antibodies of a new major allergen from grass pollen in the group I molecular weight range publication-title: J Allergy din Immunol – volume: 22 start-page: 491 year: 1992 end-page: 7 article-title: Malthiesen Group V allergens in grass pollens. IV. Similarities in ammo acid and compositions and NH terminal sequences of the Group V allergens from publication-title: Clin Exp Allergy – volume: 79 start-page: 80 year: 1991 end-page: 4 article-title: Clincal significance of aeroallergen identification in the Western Cape publication-title: South Afr Med J – volume: 55 start-page: 452 year: 1989 end-page: 63 article-title: Grass genera of Southern Africa: interactive identification and information retrieval from an automated databank publication-title: S Afr J Bot – start-page: 1 year: 1991 end-page: 2 – start-page: 8 year: 1974 end-page: 15 – volume: 43 start-page: 71 issue: Suppl 7 year: 1988 article-title: Comparison of different radioimmunoassays (RIA) and radio enzymatic assays (REA) publication-title: Allergy – volume: 133 start-page: 214 year: 1983 end-page: 9 article-title: Detection of antigens on nitrocellulose paper immunoblots with monoclonal antibodies publication-title: Anal Biochem – volume: 88 start-page: 763 year: 1991 end-page: 74 article-title: Characterization of the major allergen (Bermuda Grass) pollen Cyn d 1 publication-title: J Allergy Clin Immunol – year: 1979 – ident: e_1_2_1_11_2 – ident: e_1_2_1_3_2 doi: 10.1073/pnas.76.9.4350 – ident: e_1_2_1_4_2 doi: 10.1016/0003-2697(83)90245-2 – ident: e_1_2_1_10_2 doi: 10.1016/S0254-6299(16)31169-3 – volume: 79 start-page: 77 year: 1991 ident: e_1_2_1_8_2 article-title: Clinical characteristics of childhood asthma publication-title: South Afr Med J – volume: 79 start-page: 80 year: 1991 ident: e_1_2_1_7_2 article-title: Clincal significance of aeroallergen identification in the Western Cape publication-title: South Afr Med J – ident: e_1_2_1_14_2 doi: 10.1016/0091-6749(91)90184-P – start-page: 653 volume-title: The grasses and pastures of South Africa year: 1955 ident: e_1_2_1_15_2 – start-page: 1 volume-title: Grasses of southern Africa year: 1991 ident: e_1_2_1_9_2 – ident: e_1_2_1_2_2 – ident: e_1_2_1_13_2 doi: 10.1111/j.1365-2222.1992.tb00152.x – ident: e_1_2_1_6_2 – volume: 43 start-page: 71 issue: 7 year: 1988 ident: e_1_2_1_5_2 article-title: Comparison of different radioimmunoassays (RIA) and radio enzymatic assays (REA) publication-title: Allergy – ident: e_1_2_1_12_2 doi: 10.1016/0091-6749(89)90489-2 – reference: 8221253 - Clin Exp Allergy. 1993 Jul;23(7):537-41 |
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| Snippet | Summary
Kikuyu grass (Pennisetum clandeminum) is widely used as a grazing pasture in Africa and, although it is extensively cultivated as a lawn for sports... Kikuyu grass ( Pennisetum clandeminum ) is widely used as a grazing pasture in Africa and, although it is extensively cultivated as a lawn for sports fields... Kikuyu grass (Pennisetum clandestinum) is widely used as a grazing pasture in Africa and, although it is extensively cultivated as a lawn for sports fields and... |
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| SubjectTerms | Allergens - immunology Allergic diseases Asthma - immunology Basophils - immunology Biological and medical sciences Blotting, Western Cross Reactions Electrophoresis, Polyacrylamide Gel General aspects Histamine Release Humans Hypersensitivity, Immediate - immunology Immunoglobulin E - immunology Immunopathology Medical sciences Pennisetum clandestinum Poaceae Pollen - immunology Rhinitis - immunology |
| Title | IgE specific immune responses to an African grass (Kikuyu, Pennisetum clandestinum) |
| URI | https://api.istex.fr/ark:/67375/WNG-GSK8J0Z8-V/fulltext.pdf https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1365-2222.1993.tb00897.x https://www.ncbi.nlm.nih.gov/pubmed/7693313 https://www.proquest.com/docview/16722069 https://www.proquest.com/docview/76021430 |
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