Cloning and characterization of a chitinase (CHIT42) cDNA from the mycoparasitic fungus Trichoderma harzianum

• Published in: Current genetics Vol. 27; no. 1; p. 83
• Main Authors: Garcia, I, Lora, J.M, Cruz, J. de la, Benitez, T, Llobell, A, Pintor-Toro, J.A. (Consejo Superior de Investigaciones Cientificas, Sevilla (Spain). Inst. de Recursos Naturales y Agrobiologia)
• Format: Journal Article
• Language: English
• Published: United States 01.12.1994
• Subjects: ADN; Amino Acid Sequence; Base Sequence; CHITINASE; Chitinases - biosynthesis; Chitinases - genetics; CHITINE; CLONACION MOLECULAR; CLONAGE MOLECULAIRE; Cloning, Molecular; CONTROL BIOLOGICO; DNA, Complementary - genetics; DNA, Fungal - genetics; Enzyme Induction; Fungal Proteins - biosynthesis; Fungal Proteins - genetics; GENE; Gene Expression Regulation, Fungal; GENES; Genes, Fungal; Isoenzymes - biosynthesis; Isoenzymes - genetics; LUTTE BIOLOGIQUE; Molecular Sequence Data; PARASITISME; PARASITISMO; Protein Conformation; QUITINA; QUITINASA; SECUENCIA NUCLEOTIDICA; Sequence Alignment; Sequence Homology, Amino Acid; SEQUENCE NUCLEOTIDIQUE; Species Specificity; Trichoderma - enzymology; Trichoderma - genetics; TRICHODERMA HARZIANUM

A cDNA of Trichoderma harzianum-(chit42), coding for an endochitinase of 42 kDa, has been cloned using synthetic oligonucleotides corresponding to aminoacid sequences of the purified chitinase. The cDNA codes for a protein of 423 amino acids. Analysis of the N-terminal amino-acid sequence of the chi...