Aligner-mediated cleavage-triggered exponential amplification for sensitive detection of nucleic acids

• Published in: Talanta (Oxford) Vol. 185; pp. 141 - 145
• Main Authors: Wu, Wanghua, Fan, Hongliang, Lian, Xiang, Zhou, Jianguang, Zhang, Tao
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2018
• Subjects: Aligner-mediated cleavage; blood serum; detection limit; DNA; DNA cleavage; humans; Isothermal exponential amplification; mutation; Nucleic acids detection; Isothermal exponential amplification; DNA cleavage; Aligner-mediated cleavage; Nucleic acids detection

Exponential amplification reaction (EXPAR), as a simple and high sensitive method, holds great promise in nucleic acids detection. One major challenge in EXPAR is the generation of trigger DNA with a definite 3′-end, which now relies on fingerprinting technology. However, the requirement of differen...