Bradykinin analysis revived – A validated method for determination of its stable metabolite in whole blood by LC–MS/MS

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 947-948; pp. 139 - 144
• Main Authors: Seip, Knut F., Bjerknes, Kari C., Johansen, Harald T., Nielsen, Erik W., Landrø, Linn, Reubsaet, Léon
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2014
• Subjects: BK1–5; Blood; Bradykinin; Bradykinin - blood; Chromatography, Liquid - methods; Diseases; Enzymes; Ethyl alcohol; Hereditary angioedema; Humans; LC–MS/MS; Linearity; Metabolites; Patients; Sampling; Tandem Mass Spectrometry - methods; Validation; Vasodilator Agents - blood; Validation; BK1–5; Hereditary angioedema; LC–MS/MS; Bradykinin

•A validated method for determination of the metabolite, bradykinin 1–5 (BK1–5), has been developed.•The validation confirms good linearity, repeatability (intra-run), reproducibility (inter-run), accuracy, and recovery.•Increased BK1–5 levels during hereditary angioedema attacks were measured. Inve...