Modulation of Pteroylpolyglutamate Concentration and Length in Response to Altered Folate Nutrition in a Comprehensive Range of Rat Tissues

• Published in: The Journal of nutrition Vol. 120; no. 5; pp. 476 - 484
• Main Authors: Ward, Gregory J., Nixon, Peter F.
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 01.05.1990; American Society for Nutritional Sciences
• Subjects: Animals; Biological and medical sciences; Diet; Electrophoresis, Polyacrylamide Gel; Feeding. Feeding behavior; folate deficiency; folate excess; Folic Acid - administration & dosage; Folic Acid - analogs & derivatives; Folic Acid - analysis; Folic Acid - pharmacokinetics; Folic Acid Deficiency - metabolism; Fundamental and applied biological sciences. Psychology; gamma-Glutamyl Hydrolase; Lactobacillus casei - metabolism; Male; Molecular Weight; Nutritional Status; pteroylpolyglutamates; Pteroylpolyglutamic Acids - analysis; Rats; Rats, Inbred Strains; Tissue Distribution; Vertebrates: anatomy and physiology, studies on body, several organs or systems; pteroylpolyglutamates; folate deficiency; rats; folate excess; Vertebrata; Mammalia; Deficient diet; Rat; Rodentia; Vitamin; Folic acid; Feeding

For a range of rat tissue extracts, the concentrations of total folates and of short-chain pteroylpolyglutamates were assayed by Lactobacillus casel with and without conjugase treatment, respectively, and the concentration and chain length of H4Pte-Glnn and 5,10-CH2-H4PteGlnn together were assayed a...