Constitutive Expression of Protective Antigen Gene of Bacillus anthracis in Escherichia coli

• Published in: Biochemical and biophysical research communications Vol. 283; no. 2; pp. 308 - 315
• Main Authors: Chauhan, Vibha, Singh, Aparna, Waheed, S.Mohsin, Singh, Samer, Bhatnagar, Rakesh
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 04.05.2001
• Subjects: Animals; Anthrax - etiology; Antigens, Bacterial - biosynthesis; Antigens, Bacterial - genetics; Antigens, Bacterial - isolation & purification; Bacillus anthracis; Bacillus anthracis - genetics; Bacillus anthracis - immunology; Bacillus anthracis - pathogenicity; Bacterial Toxins - biosynthesis; Bacterial Toxins - genetics; Bacterial Toxins - immunology; Biotechnology; Carrier Proteins - metabolism; edema factor; Escherichia coli; Escherichia coli - genetics; Gene Expression; Humans; lethal factor; Plasmids - genetics; Promoter Regions, Genetic; protective antigen; Receptors, Cell Surface - metabolism; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; Recombinant Proteins - immunology; T-Phages - genetics

The fatal bacterial infection caused by inhalation of the Bacillus anthracis spores results from the synthesis of protein toxins—protective antigen (PA), lethal factor (LF), and edema factor (EF)—by the bacterium. PA is the target-cell binding protein and is common to the two effector molecules, LF...