Defective Autophagy in Diabetic Retinopathy

• Published in: Investigative ophthalmology & visual science Vol. 57; no. 10; pp. 4356 - 4366
• Main Authors: Lopes de Faria, Jacqueline M, Duarte, Diego A, Montemurro, Chiara, Papadimitriou, Alexandros, Consonni, Sílvio Roberto, Lopes de Faria, José B
• Format: Journal Article
• Language: English
• Published: United States 01.08.2016
• Subjects: Animals; Apoptosis; Autophagy - drug effects; Autophagy - physiology; Blotting, Western; Cells, Cultured; Diabetes Mellitus, Experimental; Diabetic Retinopathy - genetics; Diabetic Retinopathy - metabolism; Diabetic Retinopathy - pathology; Ependymoglial Cells - metabolism; Ependymoglial Cells - ultrastructure; Gene Expression Regulation; Glucose - pharmacology; Microscopy, Electron, Transmission; Oxidative Stress; Rats; Retina - metabolism; Retina - pathology; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; Sequestosome-1 Protein - biosynthesis; Sequestosome-1 Protein - genetics; Sweetening Agents - pharmacology

Müller cells (MCs) are a major source of VEGF in diabetic retinopathy (DR). Vascular endothelial growth factor is the main therapeutic target for treating DR. This study aimed to investigate whether autophagy is involved in MC response under high glucose (HG). Rat retinal Müller cells (rMCs) were ex...