Effects of Lidocaine and Ropivacaine on Gastric Cancer Cells Through Down-regulation of ERK1/2 Phosphorylation In Vitro

• Published in: Anticancer research Vol. 38; no. 12; pp. 6729 - 6735
• Main Authors: YANG, WENJING, CAI, JUN, ZHANG, HUIMING, WANG, GUYAN, JIANG, WENGUO
• Format: Journal Article
• Language: English
• Published: Greece International Institute of Anticancer Research 01.12.2018
• Subjects: Anesthetics; Anesthetics, Local - pharmacology; Apoptosis; Cancer; Cancer therapies; Cell adhesion & migration; Cell cycle; Cell growth; Cell Line, Tumor; Cell migration; Cell proliferation; Down-regulation; Down-Regulation - drug effects; Drug dosages; Electric cells; Extracellular signal-regulated kinase; Gastric cancer; Gentian violet; Humans; Kinases; Lidocaine; Lidocaine - pharmacology; MAP Kinase Signaling System - drug effects; Metastasis; Mitogen-Activated Protein Kinase 1 - metabolism; Mitogen-Activated Protein Kinase 3 - metabolism; Phosphorylation; Phosphorylation - drug effects; Proteins; Ropivacaine; Ropivacaine - pharmacology; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; Tumor cell lines; Variance analysis; United Kingdom > UK; United States > US; local anaesthetic; ropivacaine; Gastric carcinoma; proliferation; lidocaine
• DOI: 10.21873/anticanres.13042
• ISSN: 0250-7005; 1791-7530

Lidocaine and ropivacaine have been widely used in gastric cancer surgery. In recent years, lidocaine and ropivacaine have attracted increasing attention in cancer research, whilst effects of lidocaine and ropivacaine on gastric cancer cells have not been investigated before. This study explored the effect of lidocaine and ropivacaine on AGS and HGC-27 gastric cancer cells. AGS and HGC-27 cells were incubated with lidocaine or ropivacaine at concentrations of 10, 100 and 1 mM. At 24, 48 and 72 h after treatment, proliferation and invasion were evaluated by crystal violet assay and transwell invasion assay. Electric cell-substrate impedance sensing was applied to measure the migration of cancer cells. Phosphorylation status of extracellular-regulated protein kinases (ERK1/2) was evaluated by western blot analysis. Lidocaine (1 mM) and ropivacaine (1 mM) significantly inhibited the proliferation of AGS and HG-27 cells, but had no significant effects on invasion. Lidocaine (1 mM) and ropivacaine (1 mM) also inhibited the migration of AGS and HGC-27cells. After treatment with lidocaine (1 mM) or ropivacaine (1 mM) for 24 h, the phosphorylation of ERK1/2 in AGS and HGC-27 cells was reduced. Lidocaine at a clinically relevant concentration (10 μM), and ropivacaine at 1 mM inhibited the proliferation of gastric cancer cell lines by down-regulation of p-ERK1/2. The migration of HGC-27 cells, rather than AGS cells was more obviously inhibited by lidocaine (1 mM) and ropivacaine (1 mM). This research is easy to implement, and lays a foundation for the future research of local anaesthetics in cancer.