Porous structured cellulose microsphere acts as biosensor for glucose detection with “signal-and-color” output

• Published in: Carbohydrate polymers Vol. 205; pp. 295 - 301
• Main Authors: Li, Wei, Zhang, Li, Li, Qi, Wang, Shenggao, Luo, Xiaogang, Deng, Hongbing, Liu, Shilin
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2019
• Subjects: Biosensor; biosensors; Cellulose; color; crosslinking; glucose; Glucose detection; glucose oxidase; microgels; microparticles; Plasma grafting; starch; Glucose detection; Plasma grafting; Biosensor; Cellulose
• ISSN: 0144-8617; 1879-1344; 1879-1344
• DOI: 10.1016/j.carbpol.2018.10.084

•GOx was introduced into spider webs structured cellulose microgel.•The mocrogels had colorimetric responsivity to glucose.•The dual-color glucose probe provided an alternative color choice. In order to develop a biosensor based on porous structured cellulose microspheres for glucose detection with “signal-and-color” output, in this work, active group carboxyl was introduced to cellulose matrix by using plasma technology, and then glucose oxidase (GOx) was chemically immobilized through EDC-NHS cross-linking reaction. The cellulose microgels containing 21.28 mg/g of enzymes exhibited a fast response to 0.003 M glucose within only 4 min. As for detecting subject with a lower concentration of glucose, the probe still worked. When the concentration of glucose solution was 0.005 M, it took only 2 min that the reaction mixture changed from colorless to yellow. By the introduction of starch, the reaction mixture presented as amaranth color. Besides, the porous-structured substrate and the facile plasma technology were also promising for constructing enzyme-driven catalytic systems with enhanced performance.