Inducible regulating homologous recombination enables precise genome editing in Pichia pastoris without perturbing cellular fitness - Bai, Fan; Cai, Peng; Yao, Lun; Shen, Yiwei; Li, Yunxia; Zhou, Yongjin J. (Trends in biotechnology (Regular ed.), 2025)
Keywords: adverse effects; Biosynthesis; biotechnology; Cell cycle; Cell growth; CRISPR; CRISPR-Cas9; dynamic regulation; Editing; Efficiency; fatty alcohols; Fitness; Fungal Proteins - genetics; Fungal Proteins - metabolism; Gene deletion; Gene Editing - methods; Gene Expression Regulation, Fungal; Genes; Genetic engineering; genome assembly; Genome editing; Genomes; Glucose; Homologous recombination; Homologous Recombination - genetics; Industrial applications; Internal Medicine; Kinases; Komagataella pastoris; methanol; Natural products; Pichia - genetics; Pichia pastoris; Proteins; Rad52 DNA Repair and Recombination Protein - genetics; Rad52 protein; RNA polymerase; Saccharomycetales - genetics; tetracycline; Yeast; yeasts; dynamic regulation; genome editing; Komagataella pastoris; Pichia pastoris; homologous recombination; CRISPR-Cas9