Clinically Relevant Expansion of Hematopoietic Stem Cells with Conserved Function in a Single-Use, Closed-System Bioprocess

• Published in: Biology of blood and marrow transplantation Vol. 12; no. 10; pp. 1020 - 1030
• Main Authors: Madlambayan, Gerard J., Rogers, Ian, Purpura, Kelly A., Ito, Caryn, Yu, Mei, Kirouac, Daniel, Casper, Robert F., Zandstra, Peter W.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2006
• Subjects: Animals; B-Lymphocyte Subsets - cytology; B-Lymphocyte Subsets - immunology; Bioprocess development; Cell Culture Techniques - instrumentation; Cell Culture Techniques - methods; Cell Differentiation; Cell Lineage; Cell Separation - methods; Cells, Cultured - cytology; Colony-Forming Units Assay; Cord blood; Cord Blood Stem Cell Transplantation - methods; Fetal Blood - cytology; Graft Survival; Hematopoiesis; Hematopoietic Stem Cells - cytology; Humans; Immunophenotyping; Infant, Newborn; Killer Cells, Natural - cytology; Killer Cells, Natural - immunology; Lymphocytes - cytology; Lymphocytes - immunology; Mice; Mice, Inbred NOD; Mice, SCID; Radiation Chimera; Stem cell expansion; T-Lymphocyte Subsets - cytology; T-Lymphocyte Subsets - immunology; Transplantation, Heterologous; Stem cell expansion; Bioprocess development; Cord blood; Hematopoiesis

The clinical potential of umbilical cord blood-derived stem and progenitor cells has been demonstrated in various animal and human transplantation studies. However, the need for increased numbers of appropriate umbilical cord blood-derived cells continues to limit the development and success of thes...