Determination of inosine 5′-monophosphate dehydrogenase activity in red blood cells of thiopurine-treated patients using HPLC

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1044-1045; pp. 194 - 199
• Main Authors: Beringer, Audrey, Citterio-Quentin, Antony, Otero, Rebeca Obenza, Gustin, Clémence, Clarke, Rebecca, Salvi, Jean-Paul, Boulieu, Roselyne
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.02.2017
• Subjects: Adolescent; Adult; adults; Aged; anion exchange; Child; Child, Preschool; Chromatography, High Pressure Liquid - methods; disease control; drugs; erythrocytes; Erythrocytes - enzymology; Erythrocytes - metabolism; Female; genetic variation; graft rejection; high performance liquid chromatography; Humans; hydrophobicity; IMP Dehydrogenase - analysis; IMP Dehydrogenase - metabolism; IMPDH; inosine monophosphate; Inosine monophosphate dehydrogenase; Linear Models; Male; metabolism; Middle Aged; patients; Phenotype; Purines - analysis; Purines - metabolism; Red blood cells; Reproducibility of Results; Ribonucleotides - analysis; Ribonucleotides - metabolism; therapeutics; thiopurine S-methyltransferase; Thiopurines; Young Adult; Inosine monophosphate dehydrogenase; 6-MP; TPMT; DTT; meTIMP; RBCs; AZA; 6-TGN; IBD; Thiopurines; IMP; Phenotype; Red blood cells; IMPDH; 6-MeMPN; XMP; β-NAD; ITPA; MPA

Thiopurine drugs are commonly used in immune diseases and to a lesser extent, in transplant rejection prophylaxis: however interindividual variability in drug response and in the occurrence of adverse events is observed. Genetic variation in thiopurine S-methyltransferase (TPMT) doesn’t completely e...