Chitosan nanoparticles loaded with clove essential oil: Characterization, antioxidant and antibacterial activities

[Display omitted] •Clove essential oil (CEO) was encapsulated into chitosan nanoparticles.•FTIR, X-ray diffraction and DSC confirmed the successful loading of CEO.•Sizes of CEO-loaded nanoparticles ranged from 223 to 444 nm.•Encapsulation improved the antioxidant and antibacterial activity of CEO. O...

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Published inCarbohydrate polymers Vol. 236; p. 116075
Main Authors Hadidi, Milad, Pouramin, Shiva, Adinepour, Fateme, Haghani, Shaghayegh, Jafari, Seid Mahdi
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 15.05.2020
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Abstract [Display omitted] •Clove essential oil (CEO) was encapsulated into chitosan nanoparticles.•FTIR, X-ray diffraction and DSC confirmed the successful loading of CEO.•Sizes of CEO-loaded nanoparticles ranged from 223 to 444 nm.•Encapsulation improved the antioxidant and antibacterial activity of CEO. One of the recent trends in the food industry is application of natural antioxidant/antimicrobial agents. In this study, essential oil of clove buds was extracted and encapsulated in chitosan nanoparticles using a two-step technique of emulsion-ionic gelation. A good retention rate (55.8–73.4 %) of clove essential oil (CEO) loaded in chitosan nanoparticles was confirmed. Also, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analyses revealed the success of CEO encapsulation. Scanning electron microscopy (SEM) images illustrated regular distribution and spherical shape of nanoparticles with a size range of 223−444 nm. The antioxidant activity of CEO-loaded chitosan nanoparticles was higher than free CEO. Similarly, CEO-loaded chitosan nanoparticles had a high antibacterial activity against L. monocytogenes and S. aureus (inhibition halo diameter of 4.80–4.78 cm). This technique could improve the efficiency of CEO in food products and a delivery system for novel applications such as active packaging.
AbstractList One of the recent trends in the food industry is application of natural antioxidant/antimicrobial agents. In this study, essential oil of clove buds was extracted and encapsulated in chitosan nanoparticles using a two-step technique of emulsion-ionic gelation. A good retention rate (55.8-73.4 %) of clove essential oil (CEO) loaded in chitosan nanoparticles was confirmed. Also, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analyses revealed the success of CEO encapsulation. Scanning electron microscopy (SEM) images illustrated regular distribution and spherical shape of nanoparticles with a size range of 223-444 nm. The antioxidant activity of CEO-loaded chitosan nanoparticles was higher than free CEO. Similarly, CEO-loaded chitosan nanoparticles had a high antibacterial activity against L. monocytogenes and S. aureus (inhibition halo diameter of 4.80-4.78 cm). This technique could improve the efficiency of CEO in food products and a delivery system for novel applications such as active packaging.
One of the recent trends in the food industry is application of natural antioxidant/antimicrobial agents. In this study, essential oil of clove buds was extracted and encapsulated in chitosan nanoparticles using a two-step technique of emulsion-ionic gelation. A good retention rate (55.8–73.4 %) of clove essential oil (CEO) loaded in chitosan nanoparticles was confirmed. Also, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analyses revealed the success of CEO encapsulation. Scanning electron microscopy (SEM) images illustrated regular distribution and spherical shape of nanoparticles with a size range of 223−444 nm. The antioxidant activity of CEO-loaded chitosan nanoparticles was higher than free CEO. Similarly, CEO-loaded chitosan nanoparticles had a high antibacterial activity against L. monocytogenes and S. aureus (inhibition halo diameter of 4.80–4.78 cm). This technique could improve the efficiency of CEO in food products and a delivery system for novel applications such as active packaging.
[Display omitted] •Clove essential oil (CEO) was encapsulated into chitosan nanoparticles.•FTIR, X-ray diffraction and DSC confirmed the successful loading of CEO.•Sizes of CEO-loaded nanoparticles ranged from 223 to 444 nm.•Encapsulation improved the antioxidant and antibacterial activity of CEO. One of the recent trends in the food industry is application of natural antioxidant/antimicrobial agents. In this study, essential oil of clove buds was extracted and encapsulated in chitosan nanoparticles using a two-step technique of emulsion-ionic gelation. A good retention rate (55.8–73.4 %) of clove essential oil (CEO) loaded in chitosan nanoparticles was confirmed. Also, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analyses revealed the success of CEO encapsulation. Scanning electron microscopy (SEM) images illustrated regular distribution and spherical shape of nanoparticles with a size range of 223−444 nm. The antioxidant activity of CEO-loaded chitosan nanoparticles was higher than free CEO. Similarly, CEO-loaded chitosan nanoparticles had a high antibacterial activity against L. monocytogenes and S. aureus (inhibition halo diameter of 4.80–4.78 cm). This technique could improve the efficiency of CEO in food products and a delivery system for novel applications such as active packaging.
One of the recent trends in the food industry is application of natural antioxidant/antimicrobial agents. In this study, essential oil of clove buds was extracted and encapsulated in chitosan nanoparticles using a two-step technique of emulsion-ionic gelation. A good retention rate (55.8-73.4 %) of clove essential oil (CEO) loaded in chitosan nanoparticles was confirmed. Also, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analyses revealed the success of CEO encapsulation. Scanning electron microscopy (SEM) images illustrated regular distribution and spherical shape of nanoparticles with a size range of 223-444 nm. The antioxidant activity of CEO-loaded chitosan nanoparticles was higher than free CEO. Similarly, CEO-loaded chitosan nanoparticles had a high antibacterial activity against L. monocytogenes and S. aureus (inhibition halo diameter of 4.80-4.78 cm). This technique could improve the efficiency of CEO in food products and a delivery system for novel applications such as active packaging.One of the recent trends in the food industry is application of natural antioxidant/antimicrobial agents. In this study, essential oil of clove buds was extracted and encapsulated in chitosan nanoparticles using a two-step technique of emulsion-ionic gelation. A good retention rate (55.8-73.4 %) of clove essential oil (CEO) loaded in chitosan nanoparticles was confirmed. Also, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analyses revealed the success of CEO encapsulation. Scanning electron microscopy (SEM) images illustrated regular distribution and spherical shape of nanoparticles with a size range of 223-444 nm. The antioxidant activity of CEO-loaded chitosan nanoparticles was higher than free CEO. Similarly, CEO-loaded chitosan nanoparticles had a high antibacterial activity against L. monocytogenes and S. aureus (inhibition halo diameter of 4.80-4.78 cm). This technique could improve the efficiency of CEO in food products and a delivery system for novel applications such as active packaging.
ArticleNumber 116075
Author Adinepour, Fateme
Jafari, Seid Mahdi
Hadidi, Milad
Pouramin, Shiva
Haghani, Shaghayegh
Author_xml – sequence: 1
  givenname: Milad
  surname: Hadidi
  fullname: Hadidi, Milad
  organization: Department of Food Technology, University of Lleida, Lleida, Spain
– sequence: 2
  givenname: Shiva
  surname: Pouramin
  fullname: Pouramin, Shiva
  organization: Department of Food Science and Technology, Khazar University, Mazandaran, Iran
– sequence: 3
  givenname: Fateme
  surname: Adinepour
  fullname: Adinepour, Fateme
  organization: Department of Food Materials and Process Design Engineering, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
– sequence: 4
  givenname: Shaghayegh
  surname: Haghani
  fullname: Haghani, Shaghayegh
  organization: Department of Food Science and Technology, Khazar University, Mazandaran, Iran
– sequence: 5
  givenname: Seid Mahdi
  orcidid: 0000-0001-6877-9549
  surname: Jafari
  fullname: Jafari, Seid Mahdi
  email: smjafari@gau.ac.ir
  organization: Department of Food Materials and Process Design Engineering, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
BackLink https://www.ncbi.nlm.nih.gov/pubmed/32172888$$D View this record in MEDLINE/PubMed
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Keywords Nanoparticles
Antibacterial activity
Chitosan
Nanoencapsulation
Clove essential oil
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Snippet [Display omitted] •Clove essential oil (CEO) was encapsulated into chitosan nanoparticles.•FTIR, X-ray diffraction and DSC confirmed the successful loading of...
One of the recent trends in the food industry is application of natural antioxidant/antimicrobial agents. In this study, essential oil of clove buds was...
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SubjectTerms Antibacterial activity
antibacterial properties
antioxidant activity
antioxidants
calorimetry
Chitosan
Clove essential oil
cloves
electron microscopy
encapsulation
essential oils
food industry
Fourier transform infrared spectroscopy
gelation
Nanoencapsulation
Nanoparticles
X-ray diffraction
Title Chitosan nanoparticles loaded with clove essential oil: Characterization, antioxidant and antibacterial activities
URI https://dx.doi.org/10.1016/j.carbpol.2020.116075
https://www.ncbi.nlm.nih.gov/pubmed/32172888
https://www.proquest.com/docview/2377684305
https://www.proquest.com/docview/2440697993
Volume 236
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