Chitosan nanoparticles loaded with clove essential oil: Characterization, antioxidant and antibacterial activities

• Published in: Carbohydrate polymers Vol. 236; p. 116075
• Main Authors: Hadidi, Milad, Pouramin, Shiva, Adinepour, Fateme, Haghani, Shaghayegh, Jafari, Seid Mahdi
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.05.2020
• Subjects: Antibacterial activity; antibacterial properties; antioxidant activity; antioxidants; calorimetry; Chitosan; Clove essential oil; cloves; electron microscopy; encapsulation; essential oils; food industry; Fourier transform infrared spectroscopy; gelation; Nanoencapsulation; Nanoparticles; X-ray diffraction; Nanoparticles; Antibacterial activity; Chitosan; Nanoencapsulation; Clove essential oil
• ISSN: 0144-8617; 1879-1344; 1879-1344
• DOI: 10.1016/j.carbpol.2020.116075

[Display omitted] •Clove essential oil (CEO) was encapsulated into chitosan nanoparticles.•FTIR, X-ray diffraction and DSC confirmed the successful loading of CEO.•Sizes of CEO-loaded nanoparticles ranged from 223 to 444 nm.•Encapsulation improved the antioxidant and antibacterial activity of CEO. One of the recent trends in the food industry is application of natural antioxidant/antimicrobial agents. In this study, essential oil of clove buds was extracted and encapsulated in chitosan nanoparticles using a two-step technique of emulsion-ionic gelation. A good retention rate (55.8–73.4 %) of clove essential oil (CEO) loaded in chitosan nanoparticles was confirmed. Also, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analyses revealed the success of CEO encapsulation. Scanning electron microscopy (SEM) images illustrated regular distribution and spherical shape of nanoparticles with a size range of 223−444 nm. The antioxidant activity of CEO-loaded chitosan nanoparticles was higher than free CEO. Similarly, CEO-loaded chitosan nanoparticles had a high antibacterial activity against L. monocytogenes and S. aureus (inhibition halo diameter of 4.80–4.78 cm). This technique could improve the efficiency of CEO in food products and a delivery system for novel applications such as active packaging.