One-step reverse transcriptase-free miRNA detection system and its application for detection of gastrointestinal cancers

• Published in: Talanta (Oxford) Vol. 278; p. 126457
• Main Authors: Zhao, Guodong, Xue, Ying, Dai, Yanmiao, Zhou, Xiaojin, Li, Hui, Sheng, Guangsen, Xu, Hongwei, Chen, Ying
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2024
• Subjects: blood serum; cost effectiveness; detection limit; disease diagnosis; DNA-directed DNA polymerase; Gastrointestinal cancers; Gastrointestinal Neoplasms - diagnosis; Gastrointestinal Neoplasms - genetics; gastrointestinal system; genes; Humans; Limit of Detection; microRNA; MicroRNAs - analysis; MicroRNAs - blood; MicroRNAs - genetics; miRNA; OSMOS-qPCR; Real-Time Polymerase Chain Reaction; Reverse transcriptase-free; Taq Polymerase - metabolism; miRNA; Gastrointestinal cancers; OSMOS-qPCR; Reverse transcriptase-free

MicroRNAs (miRNAs) play pivotal roles in gene regulation and their dysregulation is implicated in various diseases, including cancer. Current methods for miRNA analysis often involve complex procedures and high costs, limiting their clinical utility. Therefore, there is a critical need for the devel...