Hydroxyeicosatetraenoic acid metabolism in cultured human skin fibroblasts : evidence for peroxisomal β-oxidation

• Published in: The Journal of clinical investigation Vol. 85; no. 4; pp. 1173 - 1181
• Main Authors: GORDON, J. A, FIGARD, P. H, SPECTOR, A. A
• Format: Journal Article
• Language: English
• Published: Ann Arbor, MI American Society for Clinical Investigation 01.04.1990
• Subjects: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; Biological and medical sciences; Cells, Cultured; Fatty Acids - metabolism; Fibroblasts - metabolism; Humans; Hydroxyeicosatetraenoic Acids - metabolism; Lipid Metabolism; Medical sciences; Metabolic diseases; Microbodies - metabolism; Oxidation-Reduction; Zellweger Syndrome - metabolism; Peroxisome; Metabolic diseases; Lipids; Skin disease; Oxidation; Fibroblast

To determine whether the peroxisome is responsible for hydroxyeicosatetraenoic acid (HETE) oxidation, 12- and 15-HETE oxidation was measured in normal and peroxisomal deficient skin fibroblasts from patients with Zellweger's (cerebrohepatorenal) syndrome. When incubated for 1 h with normal fibr...