The Hypoxic Microenvironment Induces Stearoyl-CoA Desaturase-1 Overexpression and Lipidomic Profile Changes in Clear Cell Renal Cell Carcinoma

Clear cell renal cell carcinoma (ccRCC) is the most common histological subtype of renal cell carcinoma (RCC). It is characterized by a high cell proliferation and the ability to store lipids. Previous studies have demonstrated the overexpression of enzymes associated with lipid metabolism, includin...

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Published inCancers Vol. 13; no. 12; p. 2962
Main Authors Melana, Juan Pablo, Mignolli, Francesco, Stoyanoff, Tania, Aguirre, María V., Balboa, María A., Balsinde, Jesús, Rodríguez, Juan Pablo
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 13.06.2021
MDPI
Subjects
Apoptosis
Cell growth
Cell proliferation
Chromatography
Clear cell-type renal cell carcinoma
Desaturase
Fatty acids
Flow cytometry
Hypoxia
Kidney cancer
Lipid composition
Lipid metabolism
Lipids
Metabolism
Metabolites
Microenvironments
Microscopy
Oleic acid
Phenotypes
Software
Stearoyl-CoA desaturase
Therapeutic applications
Tumor cells
Tumors
United States > US
Argentina
Spain
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Abstract Clear cell renal cell carcinoma (ccRCC) is the most common histological subtype of renal cell carcinoma (RCC). It is characterized by a high cell proliferation and the ability to store lipids. Previous studies have demonstrated the overexpression of enzymes associated with lipid metabolism, including stearoyl-CoA desaturase-1 (SCD-1), which increases the concentration of unsaturated fatty acids in tumor cells. In this work, we studied the expression of SCD-1 in primary ccRCC tumors, as well as in cell lines, to determine its influence on the tumor lipid composition and its role in cell proliferation. The lipidomic analyses of patient tumors showed that oleic acid (18:1n-9) is one of the major fatty acids, and it is particularly abundant in the neutral lipid fraction of the tumor core. Using a ccRCC cell line model and in vitro-generated chemical hypoxia, we show that SCD-1 is highly upregulated (up to 200-fold), and this causes an increase in the cellular level of 18:1n-9, which, in turn, accumulates in the neutral lipid fraction. The pharmacological inhibition of SCD-1 blocks 18:1n-9 synthesis and compromises the proliferation. The addition of exogenous 18:1n-9 to the cells reverses the effects of SCD-1 inhibition on cell proliferation. These data reinforce the role of SCD-1 as a possible therapeutic target.
AbstractList Simple SummaryClear cell renal cell carcinoma (ccRCC) is characterized by a high rate of cell proliferation and an extensive accumulation of lipids. Uncontrolled cell growth usually generates areas of intratumoral hypoxia that define the tumor phenotype. In this work, we show that, under these microenvironmental conditions, stearoyl-CoA desaturase-1 is overexpressed. This enzyme induces changes in the cellular lipidomic profile, increasing the oleic acid levels, a metabolite that is essential for cell proliferation. This work supports the idea of considering stearoyl-CoA desaturase-1 as an exploitable therapeutic target in ccRCC.AbstractClear cell renal cell carcinoma (ccRCC) is the most common histological subtype of renal cell carcinoma (RCC). It is characterized by a high cell proliferation and the ability to store lipids. Previous studies have demonstrated the overexpression of enzymes associated with lipid metabolism, including stearoyl-CoA desaturase-1 (SCD-1), which increases the concentration of unsaturated fatty acids in tumor cells. In this work, we studied the expression of SCD-1 in primary ccRCC tumors, as well as in cell lines, to determine its influence on the tumor lipid composition and its role in cell proliferation. The lipidomic analyses of patient tumors showed that oleic acid (18:1n-9) is one of the major fatty acids, and it is particularly abundant in the neutral lipid fraction of the tumor core. Using a ccRCC cell line model and in vitro-generated chemical hypoxia, we show that SCD-1 is highly upregulated (up to 200-fold), and this causes an increase in the cellular level of 18:1n-9, which, in turn, accumulates in the neutral lipid fraction. The pharmacological inhibition of SCD-1 blocks 18:1n-9 synthesis and compromises the proliferation. The addition of exogenous 18:1n-9 to the cells reverses the effects of SCD-1 inhibition on cell proliferation. These data reinforce the role of SCD-1 as a possible therapeutic target.
Clear cell renal cell carcinoma (ccRCC) is the most common histological subtype of renal cell carcinoma (RCC). It is characterized by a high cell proliferation and the ability to store lipids. Previous studies have demonstrated the overexpression of enzymes associated with lipid metabolism, including stearoyl-CoA desaturase-1 (SCD-1), which increases the concentration of unsaturated fatty acids in tumor cells. In this work, we studied the expression of SCD-1 in primary ccRCC tumors, as well as in cell lines, to determine its influence on the tumor lipid composition and its role in cell proliferation. The lipidomic analyses of patient tumors showed that oleic acid (18:1n-9) is one of the major fatty acids, and it is particularly abundant in the neutral lipid fraction of the tumor core. Using a ccRCC cell line model and in vitro-generated chemical hypoxia, we show that SCD-1 is highly upregulated (up to 200-fold), and this causes an increase in the cellular level of 18:1n-9, which, in turn, accumulates in the neutral lipid fraction. The pharmacological inhibition of SCD-1 blocks 18:1n-9 synthesis and compromises the proliferation. The addition of exogenous 18:1n-9 to the cells reverses the effects of SCD-1 inhibition on cell proliferation. These data reinforce the role of SCD-1 as a possible therapeutic target.
Author Aguirre, María V.
Melana, Juan Pablo
Mignolli, Francesco
Stoyanoff, Tania
Balboa, María A.
Balsinde, Jesús
Rodríguez, Juan Pablo
AuthorAffiliation 4 Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), 28029 Madrid, Spain
1 Laboratorio de Investigaciones Bioquímicas de la Facultad de Medicina (LIBIM), Instituto de Química Básica y Aplicada del Nordeste Argentino (IQUIBA-NEA), Universidad Nacional del Nordeste, Consejo Nacional de Investigaciones Científicas y Técnicas (UNNE-CONICET), Corrientes 3400, Argentina; jpmelana@med.unne.edu.ar (J.P.M.); taniastoyanoff@gmail.com (T.S.); vikyaguirre@yahoo.com (M.V.A.)
2 Instituto de Botánica del Nordeste, Facultad de Ciencias Agrarias (UNNE-CONICET), Universidad Nacional del Nordeste, Corrientes 3400, Argentina; fmignolli80@gmail.com
3 Instituto de Biología y Genética Molecular, Consejo Superior de Investigaciones Científicas (CSIC), 47003 Valladolid, Spain; mbalboa@ibgm.uva.es
AuthorAffiliation_xml – name: 2 Instituto de Botánica del Nordeste, Facultad de Ciencias Agrarias (UNNE-CONICET), Universidad Nacional del Nordeste, Corrientes 3400, Argentina; fmignolli80@gmail.com
– name: 4 Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), 28029 Madrid, Spain
– name: 1 Laboratorio de Investigaciones Bioquímicas de la Facultad de Medicina (LIBIM), Instituto de Química Básica y Aplicada del Nordeste Argentino (IQUIBA-NEA), Universidad Nacional del Nordeste, Consejo Nacional de Investigaciones Científicas y Técnicas (UNNE-CONICET), Corrientes 3400, Argentina; jpmelana@med.unne.edu.ar (J.P.M.); taniastoyanoff@gmail.com (T.S.); vikyaguirre@yahoo.com (M.V.A.)
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Cites_doi 10.1038/srep32582
10.1038/nm.4181
10.1016/j.cmet.2013.05.017
10.18632/oncotarget.26691
10.1038/cddis.2013.444
10.1007/s13277-016-5279-4
10.1371/journal.pone.0098705
10.1016/j.ijbiomac.2020.06.106
10.1074/jbc.M111.274829
10.1038/s41419-020-2257-y
10.1016/j.chembiol.2016.04.012
10.1016/j.ygyno.2014.02.034
10.1093/carcin/bgl143
10.1158/1535-7163.MCT-13-0098
10.1534/genetics.112.143362
10.1016/j.stem.2017.02.008
10.1139/y59-099
10.1002/jcp.26572
10.18632/oncotarget.1736
10.18632/oncotarget.26706
10.3390/cancers3022462
10.1016/j.gpb.2018.07.008
10.1016/0005-2760(87)90173-1
10.2174/156652406775574604
10.1111/j.1742-4658.2012.08644.x
10.1016/j.surg.2014.03.038
10.1073/pnas.1307237110
10.1016/j.ebiom.2017.04.009
10.1016/j.ctrv.2015.07.002
10.1007/s13277-015-3451-x
10.1038/ncomms15337
10.3390/cancers11040524
10.6004/jnccn.2019.0054
10.1016/j.celrep.2018.08.015
10.1038/bjc.2015.338
10.4049/jimmunol.1003279
10.1371/journal.pone.0010696
10.1016/j.jns.2007.03.004
10.1016/j.prostaglandins.2012.03.003
10.1111/cas.12108
10.1097/00041433-200306000-00008
10.1074/jbc.M112.442491
10.3892/ol.2013.1160
10.1194/jlr.M079145
10.1038/srep28932
10.1016/j.semcancer.2020.01.003
10.3233/KCA-170008
10.1158/1541-7786.MCR-11-0126
10.1016/j.bbalip.2010.11.009
10.1371/journal.pone.0033823
10.1242/jcs.02951
10.1371/journal.pone.0011394
10.1586/14737140.2015.1038246
10.1039/C4AN01875C
10.1038/srep19665
10.1002/cam4.293
10.1038/oncsis.2015.49
10.1007/s00418-008-0449-0
10.1016/j.cll.2005.01.012
10.1371/journal.pone.0006812
10.1186/s40170-016-0146-8
10.1152/ajpcell.00153.2012
10.1371/journal.pone.0014363
10.1158/1078-0432.CCR-12-3249
10.1016/j.bbalip.2014.10.004
10.1038/ng.2904
10.1093/carcin/bgq131
10.1016/j.bmc.2017.05.016
10.4331/wjbc.v3.i8.167
10.1016/j.cub.2013.06.032
10.1111/febs.13681
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References Sankin (ref_54) 2014; 3
Koumenis (ref_63) 2006; 6
Astudillo (ref_45) 2018; 59
Qin (ref_50) 2020; 11
Peck (ref_28) 2016; 15
Okegawa (ref_55) 2017; 19
Ueda (ref_11) 2019; 10
Herms (ref_66) 2013; 23
Liu (ref_16) 2013; 5
Gao (ref_36) 2017; 8
Santos (ref_13) 2012; 279
Goutham (ref_64) 2020; 65
Igal (ref_14) 2011; 3
ref_19
ref_18
Borsi (ref_34) 2014; 5
ref_59
Marlow (ref_61) 2013; 19
Stoyanoff (ref_27) 2016; 37
Diez (ref_41) 1987; 921
ref_60
Casuscelli (ref_56) 2017; 1
Tamura (ref_70) 2019; 10
Grignon (ref_51) 2005; 25
Roongta (ref_8) 2011; 9
Fujimoto (ref_68) 2008; 130
Wang (ref_1) 2015; 37
Valdearcos (ref_48) 2011; 186
Motzer (ref_2) 2019; 17
Shinojima (ref_38) 2007; 28
Koeberle (ref_49) 2012; 287
Lazarowski (ref_31) 2007; 258
Kato (ref_62) 2006; 119
Zhang (ref_25) 2013; 104
Schaffer (ref_73) 2003; 14
Liotti (ref_15) 2018; 233
ref_26
Bligh (ref_40) 1959; 37
Chen (ref_37) 2016; 6
Jin (ref_30) 2015; 156
Ricketts (ref_52) 2014; 46
Currie (ref_58) 2013; 18
Saito (ref_53) 2016; 6
Mukherjee (ref_20) 2017; 20
Suburu (ref_21) 2012; 98
Svensson (ref_12) 2016; 22
Jiang (ref_22) 2017; 7
Liu (ref_46) 2019; 17
Abramczyk (ref_4) 2015; 140
Kamphorst (ref_72) 2013; 110
Astudillo (ref_43) 2011; 1811
Zhang (ref_57) 2012; 3
Igal (ref_6) 2010; 31
Massari (ref_24) 2015; 41
Du (ref_32) 2014; 133
Ackerman (ref_5) 2018; 24
Colavita (ref_47) 2020; 161
Jarc (ref_65) 2017; 64
Vasseur (ref_7) 2016; 5
Wang (ref_10) 2015; 15
Chiang (ref_35) 2013; 304
Noto (ref_71) 2013; 4
Guijas (ref_69) 2014; 1841
Carrillo (ref_23) 2012; 27
Kohlwein (ref_67) 2013; 193
Wu (ref_39) 2011; 54
ref_42
Chen (ref_17) 2016; 6
Micucci (ref_29) 2015; 113
Imamura (ref_75) 2017; 25
ref_3
Peck (ref_74) 2016; 4
Nishii (ref_33) 2013; 288
Guijas (ref_44) 2016; 23
Zhou (ref_9) 2013; 12
References_xml – volume: 6
  start-page: 32582
  year: 2016
  ident: ref_37
  article-title: Physapubescin selectively induces apoptosis in VHL-null renal cell carcinoma cells through down-regulation of HIF-2α and inhibits tumor growth
  publication-title: Sci. Rep.
  doi: 10.1038/srep32582
  contributor:
    fullname: Chen
– volume: 22
  start-page: 1108
  year: 2016
  ident: ref_12
  article-title: Inhibition of acetyl-CoA carboxylase suppresses fatty acid synthesis and tumor growth of non-small-cell lung cancer in preclinical models
  publication-title: Nat. Med.
  doi: 10.1038/nm.4181
  contributor:
    fullname: Svensson
– volume: 18
  start-page: 153
  year: 2013
  ident: ref_58
  article-title: Cellular fatty acid metabolism and cancer
  publication-title: Cell Metab.
  doi: 10.1016/j.cmet.2013.05.017
  contributor:
    fullname: Currie
– volume: 10
  start-page: 1637
  year: 2019
  ident: ref_11
  article-title: Prolyl isomerase Pin1 binds to and stabilizes acetyl CoA carboxylase 1 protein, thereby supporting cancer cell proliferation
  publication-title: Oncotarget
  doi: 10.18632/oncotarget.26691
  contributor:
    fullname: Ueda
– volume: 4
  start-page: e947
  year: 2013
  ident: ref_71
  article-title: Stearoyl-CoA desaturase-1 is a key factor for lung cancer-initiating cells
  publication-title: Cell Death Dis.
  doi: 10.1038/cddis.2013.444
  contributor:
    fullname: Noto
– volume: 37
  start-page: 13581
  year: 2016
  ident: ref_27
  article-title: Tumor biology of non-metastatic stages of clear cell renal cell carcinoma; overexpression of stearoyl desaturase-1, EPO/EPO-R system and hypoxia-related proteins
  publication-title: Tumor Biol.
  doi: 10.1007/s13277-016-5279-4
  contributor:
    fullname: Stoyanoff
– ident: ref_26
  doi: 10.1371/journal.pone.0098705
– volume: 161
  start-page: 836
  year: 2020
  ident: ref_47
  article-title: Multidrug resistance protein 4 (MRP4/ABCC4) is overexpressed in clear cell renal cell carcinoma (ccRCC) and is essential to regulate cell proliferation
  publication-title: Int. J. Biol. Macromol.
  doi: 10.1016/j.ijbiomac.2020.06.106
  contributor:
    fullname: Colavita
– volume: 287
  start-page: 27244
  year: 2012
  ident: ref_49
  article-title: Palmitoleate is a mitogen, formed upon stimulation with growth factors, and converted to palmitoleoyl-phosphatidylinositol
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M111.274829
  contributor:
    fullname: Koeberle
– volume: 11
  start-page: 66
  year: 2020
  ident: ref_50
  article-title: Lipid desaturation-associated endoplasmic reticulum stress regulates MYCN gene expression in hepatocellular carcinoma cells
  publication-title: Cell Death Dis.
  doi: 10.1038/s41419-020-2257-y
  contributor:
    fullname: Qin
– volume: 23
  start-page: 689
  year: 2016
  ident: ref_44
  article-title: Foamy monocytes are enriched in cis-7-hexadecenoic fatty acid, 16:1n-9, a possible biomarker for early detection of cardiovascular disease
  publication-title: Cell Chem. Biol.
  doi: 10.1016/j.chembiol.2016.04.012
  contributor:
    fullname: Guijas
– volume: 133
  start-page: 575
  year: 2014
  ident: ref_32
  article-title: Hypoxia promotes vasculogenic mimicry formation by inducing epithelial-mesenchymal transition in ovarian carcinoma
  publication-title: Gynecol. Oncol.
  doi: 10.1016/j.ygyno.2014.02.034
  contributor:
    fullname: Du
– volume: 28
  start-page: 529
  year: 2007
  ident: ref_38
  article-title: Renal cancer cells lacking hypoxia inducible factor (HIF)-1α expression maintain vascular endothelial growth factor expression through HIF-2α
  publication-title: Carcinogenesis
  doi: 10.1093/carcin/bgl143
  contributor:
    fullname: Shinojima
– volume: 12
  start-page: 2782
  year: 2013
  ident: ref_9
  article-title: ATP citrate lyase mediates resistance of colorectal cancer cells to SN38
  publication-title: Mol. Cancer Ther.
  doi: 10.1158/1535-7163.MCT-13-0098
  contributor:
    fullname: Zhou
– volume: 193
  start-page: 1
  year: 2013
  ident: ref_67
  article-title: Lipid droplets and peroxisomes: Key players in cellular lipid homeostasis or a matter of fat-store’em up or burn’em down
  publication-title: Genetics
  doi: 10.1534/genetics.112.143362
  contributor:
    fullname: Kohlwein
– volume: 20
  start-page: 291
  year: 2017
  ident: ref_20
  article-title: Unsaturated fatty acids maintain cancer cell stemness
  publication-title: Cell Stem Cell
  doi: 10.1016/j.stem.2017.02.008
  contributor:
    fullname: Mukherjee
– volume: 37
  start-page: 911
  year: 1959
  ident: ref_40
  article-title: A Rapid method of total lipid extraction and purification
  publication-title: Can. J. Biochem. Physiol.
  doi: 10.1139/y59-099
  contributor:
    fullname: Bligh
– volume: 233
  start-page: 7367
  year: 2018
  ident: ref_15
  article-title: Oleic acid promotes prostate cancer malignant phenotype via the G protein-coupled receptor FFA1/GPR40
  publication-title: J. Cell. Physiol.
  doi: 10.1002/jcp.26572
  contributor:
    fullname: Liotti
– volume: 5
  start-page: 1779
  year: 2014
  ident: ref_34
  article-title: Hypoxia inducible factor-1 alpha as a therapeutic target in multiple myeloma
  publication-title: Oncotarget
  doi: 10.18632/oncotarget.1736
  contributor:
    fullname: Borsi
– volume: 27
  start-page: 1860
  year: 2012
  ident: ref_23
  article-title: Efecto antitumoral del ácido oleico; mecanismos de acción; revisión científica
  publication-title: Nutr. Hosp.
  contributor:
    fullname: Carrillo
– volume: 10
  start-page: 1688
  year: 2019
  ident: ref_70
  article-title: Discovery of lipid biomarkers correlated with disease progression in clear cell renal cell carcinoma using desorption electrospray ionization imaging mass spectrometry
  publication-title: Oncotarget
  doi: 10.18632/oncotarget.26706
  contributor:
    fullname: Tamura
– volume: 3
  start-page: 2462
  year: 2011
  ident: ref_14
  article-title: Roles of stearoylCoA desaturase-1 in the regulation of cancer cell growth, survival and tumorigenesis
  publication-title: Cancers
  doi: 10.3390/cancers3022462
  contributor:
    fullname: Igal
– volume: 17
  start-page: 576
  year: 2019
  ident: ref_46
  article-title: Multi-omics analysis of primary cell culture models reveals genetic and epigenetic basis of intratumoral phenotypic diversity
  publication-title: Genom. Proteom. Bioinform.
  doi: 10.1016/j.gpb.2018.07.008
  contributor:
    fullname: Liu
– volume: 64
  start-page: 549
  year: 2017
  ident: ref_65
  article-title: Lipid droplet formation in hela cervical cancer cells depends on cell density and the concentration of exogenous unsaturated fatty acids
  publication-title: Acta Chim. Slov.
  contributor:
    fullname: Jarc
– volume: 921
  start-page: 82
  year: 1987
  ident: ref_41
  article-title: Ethanol induces release of arachidonic acid but not synthesis of eicosanoids in mouse peritoneal macrophages
  publication-title: Biochim. Biophys. Acta
  doi: 10.1016/0005-2760(87)90173-1
  contributor:
    fullname: Diez
– volume: 6
  start-page: 55
  year: 2006
  ident: ref_63
  article-title: ER Stress, Hypoxia Tolerance and Tumor Progression
  publication-title: Curr. Mol. Med.
  doi: 10.2174/156652406775574604
  contributor:
    fullname: Koumenis
– volume: 279
  start-page: 2610
  year: 2012
  ident: ref_13
  article-title: Lipid metabolism in cancer
  publication-title: FEBS J.
  doi: 10.1111/j.1742-4658.2012.08644.x
  contributor:
    fullname: Santos
– volume: 156
  start-page: 221
  year: 2015
  ident: ref_30
  article-title: Development of a novel neuroprotective strategy: Combined treatment with hypothermia and valproic acid improves survival in hypoxic hippocampal cells
  publication-title: Surgery
  doi: 10.1016/j.surg.2014.03.038
  contributor:
    fullname: Jin
– volume: 110
  start-page: 8882
  year: 2013
  ident: ref_72
  article-title: Hypoxic and Ras-transformed cells support growth by scavenging unsaturated fatty acids from lysophospholipids
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.1307237110
  contributor:
    fullname: Kamphorst
– volume: 19
  start-page: 31
  year: 2017
  ident: ref_55
  article-title: Intratumor heterogeneity in primary kidney cancer revealed by metabolic profiling of multiple spatially separated samples within tumors
  publication-title: EBioMedicine
  doi: 10.1016/j.ebiom.2017.04.009
  contributor:
    fullname: Okegawa
– volume: 41
  start-page: 767
  year: 2015
  ident: ref_24
  article-title: Metabolic alterations in renal cell carcinoma
  publication-title: Cancer Treat. Rev.
  doi: 10.1016/j.ctrv.2015.07.002
  contributor:
    fullname: Massari
– volume: 37
  start-page: 479
  year: 2015
  ident: ref_1
  article-title: The role of stearoyl-coenzyme A desaturase 1 in clear cell renal cell carcinoma
  publication-title: Tumour Biol.
  doi: 10.1007/s13277-015-3451-x
  contributor:
    fullname: Wang
– volume: 8
  start-page: 15337
  year: 2017
  ident: ref_36
  article-title: VHL deficiency augments anthracycline sensitivity of clear cell renal cell carcinomas by down-regulating ALDH2
  publication-title: Nat. Commun.
  doi: 10.1038/ncomms15337
  contributor:
    fullname: Gao
– ident: ref_42
  doi: 10.3390/cancers11040524
– volume: 17
  start-page: 1278
  year: 2019
  ident: ref_2
  article-title: NCCN guidelines insights: Kidney cancer, version 2.2020
  publication-title: J. Natl. Compr. Cancer Netw.
  doi: 10.6004/jnccn.2019.0054
  contributor:
    fullname: Motzer
– volume: 24
  start-page: 2596
  year: 2018
  ident: ref_5
  article-title: Triglycerides promote lipid homeostasis during hypoxic stress by balancing fatty acid saturation
  publication-title: Cell Rep.
  doi: 10.1016/j.celrep.2018.08.015
  contributor:
    fullname: Ackerman
– volume: 113
  start-page: 1178
  year: 2015
  ident: ref_29
  article-title: HIF2α is involved in the expansion of CXCR4-positive cancer stem-like cells in renal cell carcinoma
  publication-title: Br. J. Cancer
  doi: 10.1038/bjc.2015.338
  contributor:
    fullname: Micucci
– volume: 186
  start-page: 6004
  year: 2011
  ident: ref_48
  article-title: Subcellular localization and role of lipin-1 in human macrophages
  publication-title: J. Immunol.
  doi: 10.4049/jimmunol.1003279
  contributor:
    fullname: Valdearcos
– ident: ref_3
  doi: 10.1371/journal.pone.0010696
– volume: 258
  start-page: 84
  year: 2007
  ident: ref_31
  article-title: Neuronal mdr-1 gene expression after experimental focal hypoxia—A new obstacle for neuroprotection?
  publication-title: J. Neurol. Sci.
  doi: 10.1016/j.jns.2007.03.004
  contributor:
    fullname: Lazarowski
– volume: 98
  start-page: 1
  year: 2012
  ident: ref_21
  article-title: Lipids and prostate cancer
  publication-title: Prostaglandins Other Lipid Mediat.
  doi: 10.1016/j.prostaglandins.2012.03.003
  contributor:
    fullname: Suburu
– volume: 104
  start-page: 416
  year: 2013
  ident: ref_25
  article-title: Positive feedback loop and synergistic effects between hypoxia-inducible factor-2α and stearoyl-CoA desaturase-1 promote tumorigenesis in clear cell renal cell carcinoma
  publication-title: Cancer Sci.
  doi: 10.1111/cas.12108
  contributor:
    fullname: Zhang
– volume: 14
  start-page: 281
  year: 2003
  ident: ref_73
  article-title: Lipotoxicity: When tissues overeat
  publication-title: Curr. Opin. Lipidol.
  doi: 10.1097/00041433-200306000-00008
  contributor:
    fullname: Schaffer
– volume: 288
  start-page: 11877
  year: 2013
  ident: ref_33
  article-title: The soluble form of LR11 protein is a regulator of hypoxia-induced, urokinase-type plasminogen activator receptor (uPAR)-mediated adhesion of immature hematological cells
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M112.442491
  contributor:
    fullname: Nishii
– volume: 5
  start-page: 1395
  year: 2013
  ident: ref_16
  article-title: Effects of oleic acid on cell proliferation through an integrin-linked kinase signaling pathway in 786-O renal cell carcinoma cells
  publication-title: Oncol. Lett.
  doi: 10.3892/ol.2013.1160
  contributor:
    fullname: Liu
– volume: 59
  start-page: 237
  year: 2018
  ident: ref_45
  article-title: Occurrence and biological activity of palmitoleic acid isomers in phagocytic cells
  publication-title: J. Lipid Res.
  doi: 10.1194/jlr.M079145
  contributor:
    fullname: Astudillo
– volume: 6
  start-page: 28932
  year: 2016
  ident: ref_53
  article-title: Lipidomic signatures and associated transcriptomic profiles of clear cell renal cell carcinoma
  publication-title: Sci. Rep.
  doi: 10.1038/srep28932
  contributor:
    fullname: Saito
– volume: 65
  start-page: 140
  year: 2020
  ident: ref_64
  article-title: Integrating tumor hypoxic stress in novel and more adaptable strategies for cancer immunotherapy
  publication-title: Semin. Cancer Biol.
  doi: 10.1016/j.semcancer.2020.01.003
  contributor:
    fullname: Goutham
– volume: 1
  start-page: 3
  year: 2017
  ident: ref_56
  article-title: Molecular classification of renal cell carcinoma and its implication in future clinical practice
  publication-title: Kidney Cancer
  doi: 10.3233/KCA-170008
  contributor:
    fullname: Casuscelli
– volume: 9
  start-page: 1551
  year: 2011
  ident: ref_8
  article-title: Cancer cell dependence on unsaturated fatty acids implicates stearoyl-CoA desaturase as a target for cancer therapy
  publication-title: Mol. Cancer Res.
  doi: 10.1158/1541-7786.MCR-11-0126
  contributor:
    fullname: Roongta
– volume: 1811
  start-page: 97
  year: 2011
  ident: ref_43
  article-title: Influence of cellular arachidonic acid levels on phospholipid remodeling and CoA-independent transacylase activity in human monocytes and U937 cells
  publication-title: Biochim. Biophys. Acta
  doi: 10.1016/j.bbalip.2010.11.009
  contributor:
    fullname: Astudillo
– ident: ref_60
  doi: 10.1371/journal.pone.0033823
– volume: 119
  start-page: 2342
  year: 2006
  ident: ref_62
  article-title: Ubiquitin-proteasome-dependent degradation of mammalian ER stearoyl-CoA desaturase
  publication-title: J. Cell Sci.
  doi: 10.1242/jcs.02951
  contributor:
    fullname: Kato
– ident: ref_18
  doi: 10.1371/journal.pone.0011394
– volume: 15
  start-page: 667
  year: 2015
  ident: ref_10
  article-title: The acetyl-CoA carboxylase enzyme: A target for cancer therapy?
  publication-title: Expert Rev. Anticancer Ther.
  doi: 10.1586/14737140.2015.1038246
  contributor:
    fullname: Wang
– volume: 140
  start-page: 2224
  year: 2015
  ident: ref_4
  article-title: The role of lipid droplets and adipocytes in cancer. Raman imaging of cell cultures: MCF10A, MCF7, and MDA-MB-231 compared to adipocytes in cancerous human breast tissue
  publication-title: Analyst
  doi: 10.1039/C4AN01875C
  contributor:
    fullname: Abramczyk
– volume: 6
  start-page: 19665
  year: 2016
  ident: ref_17
  article-title: Stearoyl-CoA desaturase-1 mediated cell apoptosis in colorectal cancer by promoting ceramide synthesis
  publication-title: Sci. Rep.
  doi: 10.1038/srep19665
  contributor:
    fullname: Chen
– volume: 3
  start-page: 1485
  year: 2014
  ident: ref_54
  article-title: The impact of genetic heterogeneity on biomarker development in kidney cancer assessed by multiregional sampling
  publication-title: Cancer Med.
  doi: 10.1002/cam4.293
  contributor:
    fullname: Sankin
– volume: 5
  start-page: e189
  year: 2016
  ident: ref_7
  article-title: Lipid metabolic reprogramming in cancer cells
  publication-title: Oncogenesis
  doi: 10.1038/oncsis.2015.49
  contributor:
    fullname: Vasseur
– volume: 130
  start-page: 263
  year: 2008
  ident: ref_68
  article-title: Lipid droplets: A classic organelle with new outfits
  publication-title: Histochem. Cell Biol.
  doi: 10.1007/s00418-008-0449-0
  contributor:
    fullname: Fujimoto
– volume: 54
  start-page: e2899
  year: 2011
  ident: ref_39
  article-title: Induction and testing of hypoxia in cell culture
  publication-title: J. Vis. Exp.
  contributor:
    fullname: Wu
– volume: 7
  start-page: 1
  year: 2017
  ident: ref_22
  article-title: Oleic acid induces apoptosis and autophagy in the treatment of tongue squamous cell carcinomas
  publication-title: Sci. Rep.
  contributor:
    fullname: Jiang
– volume: 25
  start-page: 305
  year: 2005
  ident: ref_51
  article-title: Clear cell renal cell carcinoma
  publication-title: Clin. Lab. Med.
  doi: 10.1016/j.cll.2005.01.012
  contributor:
    fullname: Grignon
– ident: ref_19
  doi: 10.1371/journal.pone.0006812
– volume: 4
  start-page: 6
  year: 2016
  ident: ref_74
  article-title: Inhibition of fatty acid desaturation is detrimental to cancer cell survival in metabolically compromised environments
  publication-title: Cancer Metab.
  doi: 10.1186/s40170-016-0146-8
  contributor:
    fullname: Peck
– volume: 304
  start-page: 342
  year: 2013
  ident: ref_35
  article-title: Endoplasmic reticulum stress signal impairs erythropoietin production: A role for ATF4
  publication-title: Am. J. Physiol. Cell Physiol.
  doi: 10.1152/ajpcell.00153.2012
  contributor:
    fullname: Chiang
– ident: ref_59
  doi: 10.1371/journal.pone.0014363
– volume: 19
  start-page: 2368
  year: 2013
  ident: ref_61
  article-title: Stearoyl-CoA desaturase 1 is a novel molecular therapeutic target for clear cell renal cell carcinoma
  publication-title: Clin. Cancer Res.
  doi: 10.1158/1078-0432.CCR-12-3249
  contributor:
    fullname: Marlow
– volume: 1841
  start-page: 1661
  year: 2014
  ident: ref_69
  article-title: Phospholipase A2 regulation of lipid droplet formation
  publication-title: Biochim. Biophys. Acta
  doi: 10.1016/j.bbalip.2014.10.004
  contributor:
    fullname: Guijas
– volume: 46
  start-page: 214
  year: 2014
  ident: ref_52
  article-title: Intratumoral heterogeneity in kidney cancer
  publication-title: Nat. Genet.
  doi: 10.1038/ng.2904
  contributor:
    fullname: Ricketts
– volume: 31
  start-page: 1509
  year: 2010
  ident: ref_6
  article-title: Stearoyl-Coa desaturase-1: A novel key player in the mechanisms of cell proliferation, programmed cell death and transformation to cancer
  publication-title: Carcinogenesis
  doi: 10.1093/carcin/bgq131
  contributor:
    fullname: Igal
– volume: 25
  start-page: 3768
  year: 2017
  ident: ref_75
  article-title: Discovery of novel and potent stearoyl coenzyme A desaturase 1 (SCD1) inhibitors as anticancer agents
  publication-title: Bioorg. Med. Chem.
  doi: 10.1016/j.bmc.2017.05.016
  contributor:
    fullname: Imamura
– volume: 3
  start-page: 167
  year: 2012
  ident: ref_57
  article-title: Dysregulated lipid metabolism in cancer
  publication-title: World J. Biol. Chem.
  doi: 10.4331/wjbc.v3.i8.167
  contributor:
    fullname: Zhang
– volume: 23
  start-page: 1489
  year: 2013
  ident: ref_66
  article-title: Cell to cell heterogeneity in lipid droplets suggests a mechanism to reduce lipotoxicity
  publication-title: Curr. Biol.
  doi: 10.1016/j.cub.2013.06.032
  contributor:
    fullname: Herms
– volume: 15
  start-page: 2767
  year: 2016
  ident: ref_28
  article-title: Lipid Desaturation—The next step in targeting lipogenesis in cancer?
  publication-title: FEBS J.
  doi: 10.1111/febs.13681
  contributor:
    fullname: Peck
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Snippet Clear cell renal cell carcinoma (ccRCC) is the most common histological subtype of renal cell carcinoma (RCC). It is characterized by a high cell proliferation...
Simple SummaryClear cell renal cell carcinoma (ccRCC) is characterized by a high rate of cell proliferation and an extensive accumulation of lipids....
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SubjectTerms Apoptosis
Cell growth
Cell proliferation
Chromatography
Clear cell-type renal cell carcinoma
Desaturase
Fatty acids
Flow cytometry
Hypoxia
Kidney cancer
Lipid composition
Lipid metabolism
Lipids
Metabolism
Metabolites
Microenvironments
Microscopy
Oleic acid
Phenotypes
Software
Stearoyl-CoA desaturase
Therapeutic applications
Tumor cells
Tumors
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Title The Hypoxic Microenvironment Induces Stearoyl-CoA Desaturase-1 Overexpression and Lipidomic Profile Changes in Clear Cell Renal Cell Carcinoma
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