Development and validation of a method for quantification of human insulin and its synthetic analogues in plasma and post-mortem sera by LC-MS/HRMS

• Published in: Talanta (Oxford) Vol. 225; p. 122047
• Main Authors: Bottinelli, C., Nicoli, R., Bévalot, F., Cartiser, N., Roger, C., Chikh, K., Kuuranne, T., Fanton, L., Guitton, J.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2021
• Subjects: accuracy; blood serum; Chromatography, High Pressure Liquid; Chromatography, Liquid; death; degradation; detection limit; extracts; filtration; Forensic; forensic sciences; Humans; Insulin; Insulins; Liquid chromatography; Mass spectrometry; monitoring; Post-mortem; postmortem changes; precision; quantitative analysis; sampling; solid phase extraction; storage conditions; suicide; Tandem Mass Spectrometry; therapeutics; Validation; Validation; HI; ME; Post-mortem; Liquid chromatography; Forensic; Insulin; Mass spectrometry; PMI

Analysis of human insulin and its synthetic analogues is increasingly requested for clinical monitoring, for anti-doping purposes, but also for forensic cases. Indeed, insulin analogues may be abused for suicide or homicide – whence their forensic interest. Collection and storage conditions, as well...