Characterization and serotype distribution of Aggregatibacter actinomycetemcomitans: Relationship of serotypes to herpesvirus and periodontal status in Indian subjects

• Published in: Microbial pathogenesis Vol. 110; pp. 189 - 195
• Main Authors: Joshi, Vinayak M., Bhat, Kishore G., Kugaji, Manohar S., Shirahatti, Ravi
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.09.2017
• Subjects: Actinobacillus actinomycetemcomitans; Adult; Aggregatibacter actinomycetemcomitans; Aggregatibacter actinomycetemcomitans - classification; Aggregatibacter actinomycetemcomitans - genetics; Aggregatibacter actinomycetemcomitans - isolation & purification; bacteria; Chronic Periodontitis - epidemiology; Chronic Periodontitis - microbiology; Chronic Periodontitis - virology; Coinfection; Cytomegalovirus; Dental Plaque - microbiology; Dental Plaque - virology; DNA, Bacterial - analysis; DNA, Viral - analysis; Female; Gingiva; Herpesvirus; Herpesvirus 4, Human; Human betaherpesvirus 5; Human gammaherpesvirus 4; Humans; India; Male; Middle Aged; Pasteurellaceae Infections - microbiology; Periodontal Attachment Loss - microbiology; Periodontal Diseases - microbiology; Periodontal Diseases - virology; Periodontal Index; Periodontitis; Serogroup; Serotype; serotypes; Serotyping; Simplexvirus - classification; Simplexvirus - genetics; Simplexvirus - isolation & purification; virulence; viruses; Aggregatibacter actinomycetemcomitans; Serotype; Periodontitis; Herpesvirus; India
• DOI: 10.1016/j.micpath.2017.06.041
• ISSN: 0882-4010; 1096-1208

The virulence of Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) in any individual depends on the type of strain of this bacterium. To our knowledge, there have been no studies reported in Indian subjects about A. actinomycetemcomitans serotype occurrence, co-existence with herpes virus and the possible influence of such co-existence on periodontal pathology. Subjects for this study were a subset of a larger study to identify the prevalence of A. actinomycetemcomitans in chronic periodontitis. A total of 63 subjects (12 periodontally healthy and 51 with chronic periodontitis) who were positive for A. actinomycetemcomitans were serotyped for strain-level identification. The presence of Human Cytomegalovirus (CMV) and Epstein-Barr virus (EBV) was tested in subgingival plaque samples by polymerase chain reaction. All five serotypes a to e were detected. Of the samples analyzed 38.09% harbored a single serotype, 36.5% had two serotypes, 6.3% demonstrated three and 4.7% demonstrated four serotypes. None of the samples showed presence of JP2 strain. Serotypes b, c, and e were most frequently identified in these individuals (46.03%, 36.5% and 38.09% respectively). Presence of serotypes b and c and absence of serotype d was associated with increased PD and CAL. Among 63 samples analyzed, 11 samples had CMV, four samples had EBV and nine samples had both these viruses. The PD and CAL were significantly higher (p = 0.04) when a combination of CMV and one of the serotypes was present indicating a pathological role of the coexistence. Multiple serotypes are associated with chronic periodontitis in Indians, however, JP2 strains are not detectable in this cohort. Presence of multiple serotypes and a combination of any serotype with herpesvirus is associated with greater severity of the disease. •First study to explore occurrence of A. actinomycetemcomitans serotypes in Indian subjects.•The presence of serotype e at significantly higher levels in periodontal disease compared to previous studies.•Presence of multiple serotypes (up to 4) in periodontal disease.•Combination of A. actinomycetemcomitans (any serotype) with herpesvirus may lead to an increased severity of the disease.