Optimization of the magnetic bead antigen capture enzyme immuno assay for the detection of circulating anodic antigens in mixed Schistosoma infections

• Published in: Acta tropica Vol. 59; no. 3; pp. 223 - 235
• Main Authors: Ndhlovu, P., Cadman, H., Gundersen, S.G., Vennervald, B., Friis, H., Christensen, N., Mutasa, G., Haagensen, I., Chandiwana, S.K., Deelder, A.M.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.06.1995; Elsevier
• Subjects: Adolescent; Animals; Antigens, Helminth - blood; Biological and medical sciences; Child; circulating anodic antigen; Diseases caused by trematodes; Helminthic diseases; Humans; immunodiagnosis; Immunoenzyme Techniques; Infectious diseases; magnetic beads; Magnetics; MBAC-EIA; Medical sciences; Parasitic diseases; Schistosoma haematobium; Schistosoma haematobium - immunology; Schistosoma mansoni; Schistosoma mansoni - immunology; Schistosomiases; Schistosomiasis - diagnosis; Temperature; Time Factors; Tropical medicine; Zimbabwe; Africa; Schistosoma mansoni; MBAC-EIA; immunodiagnosis; circulating anodic antigen; Schistosoma haematobium; magnetic beads; Human; Plathelmintha; Parasitosis; Trematod disease; Helminthiasis; Trematoda; Infection; Schistosomiasis; Immunology; Helmintha; Diagnosis; Invertebrata; Comparative study
• ISSN: 0001-706X; 1873-6254
• DOI: 10.1016/0001-706X(95)00083-Q

In the present study, simplification and adaptation of the Magnetic Bead Antigen Capture Enzyme Immuno Assay (MBAC-EIA) technique for detection of circulating anodic antigens (CAA) under field conditions was achieved. It was shown that the assay could be performed successfully within the broad temperature range of 18–37°C. The slightly lower sensitivity observed at low temperatures could be adjusted for by prolonging the incubation period. Shaking the plate by hand was as good as automatic mechanical shaking, aspiration of the supernatant before the addition of conjugate was not necessary, and the use of whole blood and serum offered similar assay sensitivity. Furthermore incubation times could be considerably shortened without loss of sensitivity. A major advantage of the MBAC-EIA was that the beads, after elution of bound components, were found to be reusable. The study also showed that the sensitivity of the MBAC-EIA technique in diagnosis of schistosomiasis in a Zimbabwean community endemic for both urinary and intestinal schistosomiasis, was 94%.