Nanoparticles assembled from fucoidan and trimethylchitosan as anthrax vaccine adjuvant: In vitro and in vivo efficacy in comparison to CpG

• Published in: Carbohydrate polymers Vol. 236; p. 116041
• Main Authors: Tsai, Meng-hung, Chuang, Chuan-chang, Chen, Cheng-cheung, Yen, Hui-ju, Cheng, Kuang-ming, Chen, Xin-an, Shyu, Huey-fen, Lee, Chia-ying, Young, Jenn-jong, Kau, Jyh-hwa
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.05.2020
• Subjects: Anthrax vaccine; anthrax vaccines; antibodies; Cellular uptake; Cytokines; Cytotoxicity; electrolytes; flow cytometry; fluorescence microscopes; fucoidan; Fucoidan-trimethylchitosan nanoparticles; humoral immunity; Immune protection; Immune response; immunoglobulin G; interleukin-12; interleukin-4; nanoparticles; oligodeoxyribonucleotides; vaccine adjuvants; viability; Fucoidan-trimethylchitosan nanoparticles; Cellular uptake; Immune response; Cytokines; Anthrax vaccine; Immune protection; Cytotoxicity

•Positively or negatively surface-charged FUC-TMC NPs were prepared via PEC method.•Both charged NPs showed high cell viability and low cytotoxicity on L929 cell and DC.•Both charged FUC-TMC NPs deliver drugs into the cytoplasm and the nucleus.•NPs enhanced both cellular and humoral immunity, and do...