Nanoparticles assembled from fucoidan and trimethylchitosan as anthrax vaccine adjuvant: In vitro and in vivo efficacy in comparison to CpG

•Positively or negatively surface-charged FUC-TMC NPs were prepared via PEC method.•Both charged NPs showed high cell viability and low cytotoxicity on L929 cell and DC.•Both charged FUC-TMC NPs deliver drugs into the cytoplasm and the nucleus.•NPs enhanced both cellular and humoral immunity, and do...

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Published inCarbohydrate polymers Vol. 236; p. 116041
Main Authors Tsai, Meng-hung, Chuang, Chuan-chang, Chen, Cheng-cheung, Yen, Hui-ju, Cheng, Kuang-ming, Chen, Xin-an, Shyu, Huey-fen, Lee, Chia-ying, Young, Jenn-jong, Kau, Jyh-hwa
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 15.05.2020
Subjects
Anthrax vaccine
anthrax vaccines
antibodies
Cellular uptake
Cytokines
Cytotoxicity
electrolytes
flow cytometry
fluorescence microscopes
fucoidan
Fucoidan-trimethylchitosan nanoparticles
humoral immunity
Immune protection
Immune response
immunoglobulin G
interleukin-12
interleukin-4
nanoparticles
oligodeoxyribonucleotides
vaccine adjuvants
viability
Fucoidan-trimethylchitosan nanoparticles
Cellular uptake
Immune response
Cytokines
Anthrax vaccine
Immune protection
Cytotoxicity
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Abstract •Positively or negatively surface-charged FUC-TMC NPs were prepared via PEC method.•Both charged NPs showed high cell viability and low cytotoxicity on L929 cell and DC.•Both charged FUC-TMC NPs deliver drugs into the cytoplasm and the nucleus.•NPs enhanced both cellular and humoral immunity, and dominated in humoral immunity.•AVA plus NPs show 100 % A/J mice survival rate when challenged with B. anthrax spore. Fucoidan/trimethylchitosan nanoparticles (FUC-TMC-NPs) have the potential to improve the immunostimulating efficiency of anthrax vaccine adsorbed (AVA). FUC-TMC-NPs with positive (+) or negative (–) surface charges were prepared via polyelectrolyte complexation, both charged NP types permitted high viability and presented no cytotoxicity on L929, A549 and JAWS II dendritic cells. Flow cytometry measurements indicated lower (+)-FUC-TMC-NPs internalization levels than (–)-FUC-TMC-NPs, yet produced high levels of pro-inflammatory cytokines IFN-γ, IL12p40, and IL-4. Moreover, fluorescence microscope images proved that both charged NP could deliver drugs into the nucleus. In vivo studies on A/J mice showed that (+)-FUC-TMC-NPs carrying AVA triggered an efficient response with a higher IgG anti-PA antibody titer than AVA with CpG oligodeoxynucleotides, and yielded 100 % protection when challenged with the anthracis spores. Furthermore, PA-specific IgG1 and IgG2a analysis confirmed that (+)-FUC-TMC-NPs strongly stimulated humoral immunity. In conclusion, (+)-FUC-TMC-NP is promising anthrax vaccine adjuvant as an alternative to CpG.
AbstractList Fucoidan/trimethylchitosan nanoparticles (FUC-TMC-NPs) have the potential to improve the immunostimulating efficiency of anthrax vaccine adsorbed (AVA). FUC-TMC-NPs with positive (+) or negative (–) surface charges were prepared via polyelectrolyte complexation, both charged NP types permitted high viability and presented no cytotoxicity on L929, A549 and JAWS II dendritic cells. Flow cytometry measurements indicated lower (+)-FUC-TMC-NPs internalization levels than (–)-FUC-TMC-NPs, yet produced high levels of pro-inflammatory cytokines IFN-γ, IL12p40, and IL-4. Moreover, fluorescence microscope images proved that both charged NP could deliver drugs into the nucleus. In vivo studies on A/J mice showed that (+)-FUC-TMC-NPs carrying AVA triggered an efficient response with a higher IgG anti-PA antibody titer than AVA with CpG oligodeoxynucleotides, and yielded 100 % protection when challenged with the anthracis spores. Furthermore, PA-specific IgG1 and IgG2a analysis confirmed that (+)-FUC-TMC-NPs strongly stimulated humoral immunity. In conclusion, (+)-FUC-TMC-NP is promising anthrax vaccine adjuvant as an alternative to CpG.
Fucoidan/trimethylchitosan nanoparticles (FUC-TMC-NPs) have the potential to improve the immunostimulating efficiency of anthrax vaccine adsorbed (AVA). FUC-TMC-NPs with positive (+) or negative (-) surface charges were prepared via polyelectrolyte complexation, both charged NP types permitted high viability and presented no cytotoxicity on L929, A549 and JAWS II dendritic cells. Flow cytometry measurements indicated lower (+)-FUC-TMC-NPs internalization levels than (-)-FUC-TMC-NPs, yet produced high levels of pro-inflammatory cytokines IFN-γ, IL12p40, and IL-4. Moreover, fluorescence microscope images proved that both charged NP could deliver drugs into the nucleus. In vivo studies on A/J mice showed that (+)-FUC-TMC-NPs carrying AVA triggered an efficient response with a higher IgG anti-PA antibody titer than AVA with CpG oligodeoxynucleotides, and yielded 100 % protection when challenged with the anthracis spores. Furthermore, PA-specific IgG1 and IgG2a analysis confirmed that (+)-FUC-TMC-NPs strongly stimulated humoral immunity. In conclusion, (+)-FUC-TMC-NP is promising anthrax vaccine adjuvant as an alternative to CpG.Fucoidan/trimethylchitosan nanoparticles (FUC-TMC-NPs) have the potential to improve the immunostimulating efficiency of anthrax vaccine adsorbed (AVA). FUC-TMC-NPs with positive (+) or negative (-) surface charges were prepared via polyelectrolyte complexation, both charged NP types permitted high viability and presented no cytotoxicity on L929, A549 and JAWS II dendritic cells. Flow cytometry measurements indicated lower (+)-FUC-TMC-NPs internalization levels than (-)-FUC-TMC-NPs, yet produced high levels of pro-inflammatory cytokines IFN-γ, IL12p40, and IL-4. Moreover, fluorescence microscope images proved that both charged NP could deliver drugs into the nucleus. In vivo studies on A/J mice showed that (+)-FUC-TMC-NPs carrying AVA triggered an efficient response with a higher IgG anti-PA antibody titer than AVA with CpG oligodeoxynucleotides, and yielded 100 % protection when challenged with the anthracis spores. Furthermore, PA-specific IgG1 and IgG2a analysis confirmed that (+)-FUC-TMC-NPs strongly stimulated humoral immunity. In conclusion, (+)-FUC-TMC-NP is promising anthrax vaccine adjuvant as an alternative to CpG.
•Positively or negatively surface-charged FUC-TMC NPs were prepared via PEC method.•Both charged NPs showed high cell viability and low cytotoxicity on L929 cell and DC.•Both charged FUC-TMC NPs deliver drugs into the cytoplasm and the nucleus.•NPs enhanced both cellular and humoral immunity, and dominated in humoral immunity.•AVA plus NPs show 100 % A/J mice survival rate when challenged with B. anthrax spore. Fucoidan/trimethylchitosan nanoparticles (FUC-TMC-NPs) have the potential to improve the immunostimulating efficiency of anthrax vaccine adsorbed (AVA). FUC-TMC-NPs with positive (+) or negative (–) surface charges were prepared via polyelectrolyte complexation, both charged NP types permitted high viability and presented no cytotoxicity on L929, A549 and JAWS II dendritic cells. Flow cytometry measurements indicated lower (+)-FUC-TMC-NPs internalization levels than (–)-FUC-TMC-NPs, yet produced high levels of pro-inflammatory cytokines IFN-γ, IL12p40, and IL-4. Moreover, fluorescence microscope images proved that both charged NP could deliver drugs into the nucleus. In vivo studies on A/J mice showed that (+)-FUC-TMC-NPs carrying AVA triggered an efficient response with a higher IgG anti-PA antibody titer than AVA with CpG oligodeoxynucleotides, and yielded 100 % protection when challenged with the anthracis spores. Furthermore, PA-specific IgG1 and IgG2a analysis confirmed that (+)-FUC-TMC-NPs strongly stimulated humoral immunity. In conclusion, (+)-FUC-TMC-NP is promising anthrax vaccine adjuvant as an alternative to CpG.
ArticleNumber 116041
Author Tsai, Meng-hung
Chuang, Chuan-chang
Chen, Cheng-cheung
Young, Jenn-jong
Chen, Xin-an
Kau, Jyh-hwa
Cheng, Kuang-ming
Shyu, Huey-fen
Yen, Hui-ju
Lee, Chia-ying
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Keywords Fucoidan-trimethylchitosan nanoparticles
Cellular uptake
Immune response
Cytokines
Anthrax vaccine
Immune protection
Cytotoxicity
Language English
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Snippet •Positively or negatively surface-charged FUC-TMC NPs were prepared via PEC method.•Both charged NPs showed high cell viability and low cytotoxicity on L929...
Fucoidan/trimethylchitosan nanoparticles (FUC-TMC-NPs) have the potential to improve the immunostimulating efficiency of anthrax vaccine adsorbed (AVA)....
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SubjectTerms Anthrax vaccine
anthrax vaccines
antibodies
Cellular uptake
Cytokines
Cytotoxicity
electrolytes
flow cytometry
fluorescence microscopes
fucoidan
Fucoidan-trimethylchitosan nanoparticles
humoral immunity
Immune protection
Immune response
immunoglobulin G
interleukin-12
interleukin-4
nanoparticles
oligodeoxyribonucleotides
vaccine adjuvants
viability
Title Nanoparticles assembled from fucoidan and trimethylchitosan as anthrax vaccine adjuvant: In vitro and in vivo efficacy in comparison to CpG
URI https://dx.doi.org/10.1016/j.carbpol.2020.116041
https://www.ncbi.nlm.nih.gov/pubmed/32172855
https://www.proquest.com/docview/2377684066
https://www.proquest.com/docview/2440702236
Volume 236
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